A new wireless system for decentralised measurement of physiological parameters from shake flasks

Microbial Cell Factories
Antti VasalaPeter Neubauer

Abstract

Shake flasks are widely used because of their low price and simple handling. Many researcher are, however, not aware of the physiological consequences of oxygen limitation and substrate overflow metabolism that occur in shake flasks. Availability of a wireless measuring system brings the possibilities for quality control and design of cultivation conditions. Here we present a new wireless solution for the measurement of pH and oxygen from shake flasks with standard sensors, which allows data transmission over a distance of more than 100 metres in laboratory environments. This new system was applied to monitoring of cultivation conditions in shake flasks. The at-time monitoring of the growth conditions became possible by simple means. Here we demonstrate that with typical protocols E. coli shake flask cultures run into severe oxygen limitation and the medium is strongly acidified. Additionally the strength of the new system is demonstrated by continuous monitoring of the oxygen level in methanol-fed Pichia pastoris shake flask cultures, which allows the optimisation of substrate feeding for preventing starvation or methanol overfeed. 40 % higher cell density was obtained by preventing starvation phases which occur in standard sh...Continue Reading

References

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Citations

Jan 20, 2012·Applied Microbiology and Biotechnology·T GrimmP Neubauer
Nov 20, 2008·Microbial Cell Factories·Johanna Panula-PeräläPeter Neubauer
Sep 23, 2010·Microbial Cell Factories·Boris WilmesPeter Neubauer
Mar 28, 2018·Applied Microbiology and Biotechnology·Masato Takahashi, Hideki Aoyagi
Aug 25, 2009·Bioprocess and Biosystems Engineering·Konstantin SchneiderElmar Heinzle
Sep 15, 2014·Microbial Cell Factories·Cecilia DeantonioDaniele Sblattero
Nov 26, 2009·Critical Reviews in Biotechnology·S SureshI M Mishra
Oct 6, 2020·Metabolic Engineering·Matthew RienzoMichael D Leavell

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