A newly cloned pig dolichyl-phosphate mannosyl-transferase for preventing the transmission of porcine endogenous retrovirus to human cells

Transplant International : Official Journal of the European Society for Organ Transplantation
Aki YamamotoShuji Miyagawa

Abstract

Porcine endogenous retrovirus (PERV) is a major problem associated with successful clinical xenotransplantation. In our previous study, reducing the high mannose type of N-glycan content proved to be very effective in downregulating PERV infectivity. In this study, dolichyl-phosphate mannosyltransferase (D-P-M), an enzyme related to the early stages of N-linked sugar synthesis was studied. The pig cDNA of the encoding D-P-M was newly isolated. The RNA interference (siRNA) for the D-P-M was applied and transfected to PEC(Z)/PB cells, a pig endothelial cell line with the Lac Z gene and PERV-B, to reduce the levels of high mannose type N-glycans. Compared with the mock line, the temporary PEC(Z)/PB lines showed a decreased mRNA expression for pig D-P-M, and each line then showed a clear destruction of PERV infectivity to human cells in the Lac Z pseudotype assay. The PEC(Z)/PB was next transfected with pSXGH-siRNA, H1-RNA gene promoter. The established PEC(Z)/PB clones with pSXGH-siRNA clearly led to the downregulation of PERV infectivity, as evidenced by the decreased levels of the mRNA for pig D-P-M. Reducing D-P-M enzyme activity represents a potentially useful approach to address the problem of PERV infections in clinical xeno...Continue Reading

References

Mar 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·W R LeeT H Lee
Mar 1, 1997·Nature Medicine·C PatienceR A Weiss
Oct 24, 1997·Nature·P Le TissierR A Weiss
Aug 4, 2001·The Journal of Biological Chemistry·S MiyagawaN Taniguchi
Feb 9, 2002·Molecular Reproduction and Development·Hiroshi MurakamiTaroh Kinoshita
Feb 28, 2002·Journal of Virology·Beth A OldmixonClive Patience
May 13, 2003·Proceedings of the National Academy of Sciences of the United States of America·Thomas A EricssonClive Patience
Aug 23, 2003·Lancet·David K C Cooper
Oct 3, 2003·Biochemical and Biophysical Research Communications·Kenji HazamaRyota Shirakura
Aug 17, 2004·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Jay A Fishman, Clive Patience
Apr 21, 2005·The Journal of Clinical Endocrinology and Metabolism·Liangwu SunHudson H Freeze
May 3, 2005·Transplant International : Official Journal of the European Society for Organ Transplantation·Monica E WinklerUlrich Martin
May 7, 2005·Microbes and Infection·Rie WatanabeYoshiharu Matsuura
Aug 2, 2005·European Journal of Medical Genetics·E SchollenG Matthijs
Mar 28, 2006·Biochemical and Biophysical Research Communications·Katsuyoshi MatsunamiRyota Shirakura
Jun 7, 2007·American Journal of Medical Genetics. Part a·Christian KranzHudson H Freeze

❮ Previous
Next ❯

Citations

Aug 7, 2012·Surgery Today·Aki YamamotoShuji Miyagawa
Mar 5, 2013·Molecules and Cells·Yi-Deun JungHeui-Soo Kim
Aug 21, 2010·Xenotransplantation·Mårten K J Schneider, Jörg D Seebach
Jan 21, 2012·Current Opinion in Organ Transplantation·Shuji MiyagawaMasahiro Fukuzawa

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.