A newly established real-time PCR for detection of Borrelia miyamotoi in Ixodes ricinus ticks

Ticks and Tick-borne Diseases
Michael ReiterGerold Stanek

Abstract

A total of 350 ticks collected in Austria were analyzed for the presence of DNA sequences of B. miyamotoi. Three ticks gave positive results in a B. miyamotoi-specific nested PCR. Results were confirmed by sequencing the amplified glpQ gene from the positive samples. Moreover we developed a real-time PCR which unambiguously detected B. miyamotoi in all positive samples. Further genotyping of the samples found 100% identity of the 16S-23S intergenic spacer region with Swedish B. miyamotoi sequences. This is the first detection of the relapsing fever spirochete Borrelia miyamotoi in hard ticks in Austria. The results consolidate the picture of a European-wide distribution of B. miyamotoi and again underscore the need for clinical awareness to clarify possible involvement of this species in human disease.

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Citations

Jan 6, 2017·Vector Borne and Zoonotic Diseases·Sally J CutlerAna Amaro
Apr 20, 2018·The Journal of Biological Chemistry·Alexander ZwirzitzVladimir Leksa
Feb 6, 2017·Frontiers in Immunology·Brandee L Stone, Catherine A Brissette
Aug 21, 2020·Emerging Infectious Diseases·Selma TobudicHeimo Lagler
Mar 24, 2021·Emerging Infectious Diseases·Mateusz MarkowiczGerold Stanek
Jun 25, 2021·Parasitology International·Bernhard W SonnbergerHans-Peter Fuehrer

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