PMID: 9191101Jun 1, 1997Paper

A newly identified membrane protein localized exclusively in intracellular organelles of neurons

Brain Research. Molecular Brain Research
Y KadotaShigeru Taketani

Abstract

We report here cloning of the cDNA of a novel membrane protein, termed p24, which, of the eight mouse tissues tested, was found only in brain where it is localized exclusively in neurons. The cDNA encodes 196 amino acids with a molecular weight of approximately 24000. P24 contains two putative membrane spanning domains and a sequence in the hydrophilic tail homologous to the microtubule-binding domain of microtubule-associated proteins, such as TAU and MAP-2. We prepared antibodies to p24 and demonstrated that the protein is rich in nerve fibers of the cerebral cortex, anterior cerebral nuclei and hypothalamus. When neuroblastoma Neuro 2a cells were treated with retinoic acid to induce differentiation, p24 mRNA increased but the p24 protein was not detected. The protein expressed from the p24 cDNA in non-neuronal Cos-7 cells was 24 kDa in size and were localized only in lysosomes. These findings indicate that p24 is a neuron-specific membrane protein localized in intracellular organelles of highly differentiated neural cells and suggest that it may play a role in the neural organelle transport system.

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Citations

Apr 25, 2000·Brain Research. Developmental Brain Research·B M KivellJ H Miller
Nov 26, 2002·Neuroscience Research·Masasuke ArakiShigeru Taketani
Feb 27, 2001·Brain Research. Brain Research Protocols·B M KivellJ H Miller
Apr 25, 2007·Acta Oto-laryngologica·Gi Jung ImSeo Jin Kim
May 5, 2012·Mechanisms of Development·Sehresh SaleemGinger E Carney
Jan 22, 2004·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Bogusława BudziszewskaWładysław Lasoń

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