Abstract
We designed a new promoter that drives transgene expression in an exclusively neuron-specific manner. The promoter of superior cervical ganglion10 (SCG10), expressed in neurons, was further modified to enhance its neuron specificity and activity by changing its length and fusing a multiple neuronal restrictive silencer element (NRSE) to its upstream or downstream regions. The promoter, which contained 2 kb original promoter length and two extra NRSEs in its downstream region, eventually exhibited remarkable neuron specificity as well as strong activity. To further amplify the promoter activity, the promoter was introduced into a Cre recombinase (Cre)-expressing adenovirus, and subsequent combination with Cre-inducible enhanced green fluorescence protein (EGFP)-expressing adenovirus vector, which has much stronger general promoter, resulted in a remarkably strong gene expression exclusively in neuronal cells of mixed cultures and in an animal model. This system is also applicable to astrocyte-specific expression; for instance, by changing the Cre promoter cassette to an astrocyte-specific promoter. The present relatively compact promoter combined with Cre/loxP system could be useful for a wide range of transgene experiments in v...Continue Reading
References
Jul 1, 1992·Neuron·N MoriD J Anderson
Aug 1, 1990·Neuron·S Forss-PetterJ G Sutcliffe
Jun 19, 1989·Brain Research·L L HallJ J Anders
Jun 1, 1993·The European Journal of Neuroscience·C Goujet-ZalcB Zalc
Dec 30, 1994·Neuroreport·P HorellouJ Mallet
Jun 18, 1993·Cell·H GuK Rajewsky
Oct 1, 1995·Current Opinion in Neurobiology·C J Schoenherr, D J Anderson
May 1, 1997·Journal of Neurochemistry·J J RobertJ Mallet
May 27, 1997·Proceedings of the National Academy of Sciences of the United States of America·A BessisJ P Changeux
Apr 18, 1998·Proceedings of the National Academy of Sciences of the United States of America·P KallunkiF S Jones
Mar 26, 1998·Biochemical and Biophysical Research Communications·Y SatoI Saito
Aug 10, 1999·Neuroreport·K MiyaguchiN Mori
Sep 3, 1999·Nature Biotechnology·S MillecampsM Barkats
Dec 22, 1999·Gene Therapy·V NavarroG L Gal La Salle
Feb 13, 2001·Molecular and Cellular Neurosciences·S KüglerM Bähr
May 4, 2002·Neuroscience Letters·Nobuhiko OmoriFutoshi Shibasaki
Oct 3, 2002·Journal of Neurochemistry·Masaru HonmaHiroshi Kiyama
Jun 16, 2004·Journal of Neuroscience Methods·Yumi SatoTeiichi Furuichi
Citations
Oct 20, 2012·Cancer Gene Therapy·V AkerstromM B Breslin
Nov 6, 2010·Nucleic Acids Research·Yumi KanegaeIzumu Saito
Dec 4, 2012·BMC Biology·Xiayang XieJeff S Mumm
Dec 25, 2009·Rinshō shinkeigaku = Clinical neurology·Hiroshi Kiyama
Jul 17, 2015·Gene Therapy·D SimãoP M Alves
Apr 28, 2009·Advanced Drug Delivery Reviews·Jérôme BoulaireShu Wang
Feb 28, 2015·Biochemical and Biophysical Research Communications·Wiebke SchulzeHitoshi Hashimoto
Jun 20, 2008·Brain Research·Danielle E ReadAdrienne M Gorman
Mar 11, 2006·Neuroscience·E KawakitaO Shido
Jul 3, 2010·Experimental Cell Research·E Maheswara ReddyAnjali Shiras
Jul 24, 2007·Progress in Neurobiology·X NavarroAntoni Valero-Cabré
Nov 23, 2006·Human Molecular Genetics·Yasuko KitaoSatoshi Ogawa