A newly thermoactive and detergent-stable lipase from annular sea bream (Diplodus annularis): Biochemical properties

Biotechnology and Applied Biochemistry
Nabil SmichiAhmed Fendri

Abstract

A lipolytic activity was located in the annular seabream pyloric caeca, from which a digestive lipase (AsDL) was purified. Pure AsDL has an apparent molecular mass of 50 kDa. The purified lipase is thermoactive as it displays its maximal activity on short- and long-chain triacylglycerols at a temperature of 50 °C. The enzyme is alkaline resistant as it retains 90% of its maximal activity when incubated during 1 H at pH 10. No colipase was detected in the annular seabream pyloric caeca. Similar results were reported for the sardine and the gray mullet digestive systems. This is in line with the idea that colipase might have evolved in mammal animals simultaneously with the appearance of an exocrine pancreas. AsDL is a serine enzyme, like all known lipases from different origins. Interestingly, the pure lipase was found to be insensitive to Triton X-100, a synthetic detergent, addition even at a concentration as high as 12 mM. The purified enzyme has potential applications in detergent and food industry because of its thermal activity and alkaline nature.

References

Sep 10, 1992·Nature·H van TilbeurghC Cambillau
Sep 1, 1991·Journal of General Microbiology·E J GilbertC W Jones
Dec 18, 1989·Biochimica Et Biophysica Acta·Y GargouriR Verger
Jan 1, 1994·Advances in Protein Chemistry·Z S Derewenda
May 23, 1998·Annual Review of Pharmacology and Toxicology·T Satoh, M Hosokawa
Nov 26, 1999·Biochimica Et Biophysica Acta·H van TilbeurghF Carrière
Mar 23, 2000·Biochimie·A SayariY Gargouri
Nov 18, 2000·Biochimica Et Biophysica Acta·D A Six, E A Dennis
Nov 2, 2001·The Journal of Biological Chemistry·Alain RousselChristian Cambillau
Jul 2, 2002·Journal of Lipid Research·Howard Wong, Michael C Schotz
Dec 28, 1964·Annals of the New York Academy of Sciences·L ORNSTEIN
Dec 28, 1964·Annals of the New York Academy of Sciences·B J DAVIS
May 17, 2005·Methods : a Companion to Methods in Enzymology·Dean Gilham, Richard Lehner
May 24, 2006·Biotechnology Advances·Pablo Domínguez de MaríaAndrés R Alcántara
Feb 7, 2009·Colloids and Surfaces. B, Biointerfaces·Ikram JemelSofiane Bezzine
Jul 2, 2010·Fish Physiology and Biochemistry·Héctor NolascoFernando Vega-Villasante
May 3, 2011·Trends in Endocrinology and Metabolism : TEM·Ariel D Quiroga, Richard Lehner
May 10, 2011·Lipids in Health and Disease·Abir Ben BachaYassine Ben Ali
Oct 4, 2011·Biochimica Et Biophysica Acta·Ariel D Quiroga, Richard Lehner
Mar 30, 2012·Fish Physiology and Biochemistry·Nabil SmichiNabil Miled
Apr 3, 2013·International Journal of Biological Macromolecules·Nabil SmichiAhmed Fendri

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