A NIMA-related kinase, CNK4, regulates ciliary stability and length

Molecular Biology of the Cell
Dan Meng, Junmin Pan

Abstract

NIMA-related kinases (Nrks or Neks) have emerged as key regulators of ciliogenesis. In human, mutations in Nek1 and Nek8 cause cilia-related disorders. The ciliary functions of Nrks are mostly revealed by genetic studies; however, the underlying mechanisms are not well understood. Here we show that a Chlamydomonas Nrk, CNK4, regulates ciliary stability and length. CNK4 is localized to the basal body region and the flagella. The cnk4-null mutant exhibited long flagella, with formation of flagellar bulges. The flagella gradually became curled at the bulge formation site, leading to flagellar loss. Electron microscopy shows that the curled flagella involved curling and degeneration of axonemal microtubules. cnk4 mutation resulted in flagellar increases of IFT trains, as well as its accumulation at the flagellar bulges. IFT speeds were not affected, however, IFT trains frequently stalled, leading to reduced IFT frequencies. These data are consistent with a model in which CNK4 regulates microtubule dynamics and IFT to control flagellar stability and length.

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Citations

Jan 31, 2018·Molecular and Cellular Biology·Muniesh Muthaiyan ShanmugamOliver Ingvar Wagner
Mar 28, 2018·Nature Communications·Monia PorporaAntonio Feliciello
Feb 23, 2019·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Yingrui WangJunmin Pan
Feb 1, 2020·Wiley Interdisciplinary Reviews. Developmental Biology·Chase C WesleyDaniel L Levy
May 28, 2020·Molecular Biology of the Cell·Kelly M HennesseyAlexander R Paredez
Nov 12, 2021·ACS Chemical Biology·Zhijun DongJunmin Pan

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