A ninhydrin-based assay to quantitate the total protein content of tissue samples

Analytical Biochemistry
B C Starcher

Abstract

Quantitation of small tissue samples for total protein content is essential for many biochemical analyses. In this study a ninhydrin method for measuring the total protein content of tissue hydrolysates is presented. The ninhydrin reagent is stable at room temperature for up to 1 month in the ethylene glycol-sodium acetate solvent system without the requirement for a nitrogen atmosphere. The reaction was very accurate and precise, with intra- and interassay variations of less than 3% when 5 microg of protein was assayed. All proteins that were investigated contributed the same color intensity per microgram protein as bovine serum albumin. This assay was several times more sensitive than the Coomassie reaction and linear over a greater range of protein concentration.

References

Jul 1, 1975·Journal of Agricultural and Food Chemistry·N D HeidelbaughH O Wheeler
May 15, 1992·Analytical Biochemistry·A Alam
Sep 1, 1988·Analytical Biochemistry·S M GothamW R Paterson

❮ Previous
Next ❯

Citations

Feb 1, 2012·Journal of Cardiovascular Translational Research·Jessica E Wagenseil, Robert P Mecham
May 5, 2012·Molecular & Cellular Proteomics : MCP·Stephan MichalikMichael Hecker
Jul 19, 2006·Tissue Engineering·Chrysanthi WilliamsRobert T Tranquillo
Oct 21, 2011·Tissue Engineering. Part a·Jason W BjorkRobert T Tranquillo
Oct 14, 2008·American Journal of Physiology. Lung Cellular and Molecular Physiology·O Le SauxC Jourdan-Le Saux
Aug 12, 2008·American Journal of Physiology. Lung Cellular and Molecular Physiology·Barry StarcherAlexsander Hinek
Jul 15, 2011·International Journal of Nanomedicine·Gunter AlmerRuth Prassl
Sep 21, 2013·Applied Biochemistry and Biotechnology·Mai Ostergaard Haven, Henning Jørgensen
Feb 8, 2014·Photosynthesis Research·Thomas J KeelingMatthew S Kimber
Dec 11, 2013·Tissue Engineering. Part a·Liqiong GuiLaura E Niklason
Sep 1, 2014·International Journal of Food Microbiology·Natan de Jesus Pimentel-FilhoMaria Cristina Dantas Vanetti
Dec 7, 2005·Cell and Tissue Research·D F EggingJ Schalkwijk
Jun 13, 2012·Biomedical Instrumentation & Technology·Shanil P HaugenVictoria M Hitchins
Oct 8, 2011·Journal of Materials Science. Materials in Medicine·Ruirui LiuJihua Chen
Dec 1, 2009·Applied Biochemistry and Biotechnology·Maobing Tu, Jack N Saddler
Feb 19, 2014·Cellular and Molecular Bioengineering·Justin S WeinbaumRobert T Tranquillo
Jul 10, 2013·Amino Acids·Sung Ung KangGert Lubec
May 11, 2006·Experimental Lung Research·Barry Starcher, Charles H Hill
Apr 22, 2014·Journal of the American Society of Hypertension : JASH·Tu H MaiDavid Robertson
Feb 14, 2015·Proteomics. Clinical Applications·Peggy KloppotSusanne Engelmann
Mar 29, 2011·Acta Biomaterialia·Stephanie K SeidlitsChristine E Schmidt
Jan 5, 2011·Biomaterials·Jason W BjorkRobert T Tranquillo
May 14, 2010·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·A GrantP Morrow

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.