A nitrate-inducible ferredoxin in maize roots. Genomic organization and differential expression of two nonphotosynthetic ferredoxin isoproteins

Plant Physiology
T MatsumuraToshiharu Hase

Abstract

We have identified and characterized a nitrate-inducible ferredoxin (Fd) in maize (Zea mays L.) roots by structural analysis of the purified protein and by cloning of its cDNA and gene. In maize Fd isoproteins are encoded by a small multigene family, and the nitrate-inducible Fd was identified as a novel isoprotein, designated Fd VI, which differed from an Fd I to Fd V identified to date. In the roots of seedlings cultured without nitrate, Fd VI was undetectable. However, during the induction of the capacity for nitrate assimilation, the amount of Fd VI increased markedly within 24 h. Concurrently, the level of transcript for Fd VI increased, but more quickly, reaching a maximal level within 2 h with kinetics similar to those of nitrite reductase and Fd-NADP+ reductase. Fd III was constitutively expressed in roots, and no such changes at the protein and mRNA levels were observed during the nitrate induction. In the 5' flanking region of the gene for Fd VI only, we identified NIT-2 motifs, which are widely found in genes for enzymes related to nitrogen metabolism. These data indicate that Fd VI is co-induced with the previously characterized enzymes involved in nitrate assimilation, and they suggest that the novel Fd isoprotein,...Continue Reading

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Citations

Jun 22, 1999·Current Opinion in Plant Biology·M Stitt
Apr 15, 2004·Nature Reviews. Microbiology·Stuart A RalphGeoffrey I McFadden
Jun 17, 1998·The Plant Journal : for Cell and Molecular Biology·H SakakibaraT Sugiyama
Jul 10, 2009·The Journal of Biological Chemistry·Aimee M TerauchiSabeeha S Merchant
Dec 20, 2003·Plant Physiology·Guy Thomas HankeToshiharu Hase
Mar 12, 2004·Annual Review of Plant Physiology and Plant Molecular Biology·H. E. Neuhaus, M. J. Emes
May 5, 2001·Annual Review of Plant Physiology and Plant Molecular Biology·Arthur Grossman, Hideki Takahashi
Jul 11, 2014·Journal of Experimental Botany·Juan J Pierella KarlusichNéstor Carrillo
Jan 11, 2003·Biotechnology & Genetic Engineering Reviews·Don J Durzan, M Cristina Pedroso
Mar 23, 2004·Physiologia Plantarum·Renate Scheibe
May 23, 2012·Plant Biotechnology Journal·Chandra H McAllisterAllen G Good
Nov 30, 2012·Plant, Cell & Environment·Guy Hanke, Paula Mulo
Aug 11, 2015·Plant Science : an International Journal of Experimental Plant Biology·Chunmei LiPingrong Wang
Mar 13, 2017·Photosynthesis Research·Silas Busck MellorPoul Erik Jensen
Feb 6, 2017·Photosynthesis Research·Juan José Pierella Karlusich, Néstor Carrillo
May 11, 2021·Frontiers in Plant Science·Srikanth BollamRajeev Gupta

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