A nitrite microsensor for profiling environmental biofilms.

Applied and Environmental Microbiology
D De BeerM Kuhl

Abstract

A highly selective liquid membrane nitrite microsensor based on the hydrophobic ion-carrier aquocyanocobalt(III)-hepta(2-phenylethyl)-cobrynate is described. The sensor has a tip diameter of 10 to 15 (mu)m. The response is log-linear in freshwater down to 1 (mu)M NO(inf2)(sup-) and in seawater to 10 (mu)M NO(inf2)(sup-). A method is described for preparation of relatively large polyvinyl chloride (PVC)-gelled liquid membrane microsensors with a tip diameter of 5 to 15 (mu)m, having a hydrophilic coating on the tip. The coating and increased tip diameter resulted in more sturdy sensors, with a lower detection limit and a more stable signal than uncoated nitrite sensors with a tip diameter of 1 to 3 (mu)m. The coating protects the sensor membrane from detrimental direct contact with biomass and can be used for all PVC-gelled liquid membrane sensors meant for profiling microbial mats, biofilms, and sediments. Thanks to these improvements, liquid membrane sensors can now be used in complex environmental samples and in situ, e.g., in operating bioreactors. Examples of measurements in denitrifying, nitrifying, and nitrifying/denitrifying biofilms from wastewater treatment plants are shown. In all of these biofilms high nitrite concen...Continue Reading

References

Jul 1, 1987·Pflügers Archiv : European journal of physiology·D AmmannW Simon
Jul 1, 1983·Journal of Neuroscience Methods·J L MunozJ A Coles
Aug 1, 1995·Applied and Environmental Microbiology·J P CarterD J Richardson
Mar 15, 1996·Biochimica Et Biophysica Acta·C Doblander, R Lackner
Sep 1, 1988·Applied and Environmental Microbiology·N P RevsbechJ Sørensen
Feb 1, 1993·Applied and Environmental Microbiology·D de BeerS P Ottengraf
Apr 1, 1996·Applied and Environmental Microbiology·L H LarsenS J Binnerup
Jun 1, 1994·Talanta·U SchallerE Pretsch

❮ Previous
Next ❯

Citations

Mar 7, 2001·Applied and Environmental Microbiology·A GiesekeA Schramm
Aug 16, 2003·Environmental Microbiology·Dörte AltmannAndreas Schramm
Aug 18, 2005·Environmental Microbiology·Armin GiesekeDirk de Beer
May 14, 2005·Biotechnology and Bioengineering·Hisashi SatohTakuya Suzuki
Sep 4, 2008·Biotechnology and Bioengineering·Leon S Downing, Robert Nerenberg
Sep 25, 2008·Applied Microbiology and Biotechnology·Leon S Downing, Robert Nerenberg
Oct 6, 2009·Environmental Microbiology·Shinya MatsumotoMark C M van Loosdrecht
Mar 24, 2010·BMC Biology·Frank SchreiberPaul Stoodley
Jan 30, 2015·Environmental Microbiology·Judith M KlattLubos Polerecky
Sep 7, 2001·Annual Review of Microbiology·G A Kowalchuk, J R Stephen
Apr 26, 2003·Environmental Microbiology·Armin GiesekeRudolf Amann
May 5, 2005·Applied and Environmental Microbiology·Satoshi OkabeHisashi Satoh
Dec 5, 2006·Applied and Environmental Microbiology·Satoshi OkabeHisashi Satoh
Jun 27, 2003·Biotechnology and Bioengineering·Hisashi SatohSatoshi Okabe
Jan 1, 2009·Annual Review of Marine Science·Tommy S MooreGeorge W Luther
Dec 20, 2016·Measurement Science & Technology·Xiangmeng MaDarren A Lytle
Mar 25, 2020·International Journal of Environmental Research and Public Health·Hong LiuNing Ding
Jul 22, 2020·Analytical and Bioanalytical Chemistry·Giada Caniglia, Christine Kranz
Mar 7, 2003·Applied and Environmental Microbiology·Marcus A HornHarold L Drake
Oct 6, 2005·Applied and Environmental Microbiology·Rikke Louise MeyerDiane Elizabeth Allen
Feb 12, 2008·Nature Reviews. Microbiology·Philip S Stewart, Michael J Franklin
May 29, 2007·Applied and Environmental Microbiology·Tomonori KindaichiSatoshi Okabe
Nov 6, 2008·Biotechnology and Bioengineering·E S McLamoreM K Banks
Oct 16, 2010·Environmental Microbiology·Anne-Christin GirnthAntje Boetius
Apr 5, 2013·FEMS Microbiology Reviews·Michiel VosGeorge A Kowalchuk
Feb 27, 2015·Reports on Progress in Physics·Nicole BillingsKatharina Ribbeck
Dec 31, 2016·Environmental Microbiology·Dirk de BeerJudith M Klatt
Feb 24, 2001·Environmental Microbiology·A SchrammR Amann
Jun 6, 2006·Applied and Environmental Microbiology·Armin GiesekeDirk de Beer

❮ Previous
Next ❯

Related Feeds

Biofilm & Infectious Disease

Biofilm formation is a key virulence factor for a wide range of microorganisms that cause chronic infections.Here is the latest research on biofilm and infectious diseases.