A nitrocellulose membrane-based integrated microfluidic system for bacterial detection utilizing magnetic-composite membrane microdevices and bacteria-specific aptamers

Lab on a Chip
Jia-Han WuGwo-Bin Lee

Abstract

Bacteria such as Acinetobacter baumannii (AB) can cause serious infections, resulting in high mortality if not diagnosed early and treated properly; there is consequently a need for rapid and accurate detection of this bacterial species. Therefore, we developed a new, nitrocellulose-based microfluidic system featuring AB-specific aptamers capable of automating the bacterial detection process via the activity of microfluidic devices composed of magnetic-composite membranes. Electromagnets were used to actuate these microfluidic devices such that the entire diagnostic process could be conducted in the integrated microfluidic system within 40 minutes with a limit of detection as low as 450 CFU per reaction for AB. Aptamers were used to capture AB in complex samples on nitrocellulose membranes, and a simple colorimetric assay was used to estimate bacterial loads. Given the ease of use, portability, and sensitivity of this aptamer-based microfluidic system, it may hold great promise for point-of-care diagnostics.

References

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Citations

Sep 25, 2019·Journal of Separation Science·Chuchu WangHao Li
Jul 28, 2020·International Journal of Molecular Sciences·Nevina E Trunzo, Ka Lok Hong
Jan 29, 2021·Analytical and Bioanalytical Chemistry·Lin ZhuChaoyong Yang
Mar 9, 2021·Frontiers in Microbiology·Mariam Saad, Sebastien P Faucher
Nov 10, 2018·Analytical Chemistry·Yanling SongChaoyong Yang

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Methods Mentioned

BETA
enzyme-linked immunosorbent assay
SELEX
antibody assay
chip
chips

Software Mentioned

MFOLD
ImageJ
Arduino integrated development environment ( IDE )

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