A Non-Enzymatic Method to Obtain a Fat Tissue Derivative Highly Enriched in Adipose Stem Cells (ASCs) from Human Lipoaspirates: Preliminary Results

International Journal of Molecular Sciences
Francesco De FrancescoMichele Riccio

Abstract

Adipose tissue possesses phenotypic gene expression characteristics that are similar to human mesenchymal stem cells (hMSCs). Nevertheless, the multilineage potential may be inhibited, and cells may not expand adequately to satisfy the requirements of Good Manufacturing Practice (cGMP). An autologous hMSC-enriched fat product would fulfil the void from a biomedical and clinical perspective. In this study, we suggest a novel mechanism using a closed system without enzymes, additives or other modifications, which will produce non-expanded, accessible material. This decentralized fat product, unlike unprocessed lipoaspirates, adequately encloses the vascular stroma with adipocytes and stromal stalks along with their vascular channels and lumina. This fat product contained hASCs and fewer hematopoietic elements such as lipoaspirates, which were digested enzymatically according to flow cytometric investigations, and molecular analysis also showed significant hASC uniformity within the cells of the stromal vascular tissue. Moreover, the fat product produced a higher quantity of hASCs similar to hMSCs in isolation with the typical characteristics of an osteogenic, chondrogenic and adipogenic lineage. Interestingly, these properties we...Continue Reading

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Citations

May 1, 2020·International Journal of Molecular Sciences·Elena Dai PrèAndrea Sbarbati
Nov 8, 2018·International Journal of Molecular Sciences·Chiara ArgentatiSabata Martino
Apr 25, 2020·Current Reviews in Musculoskeletal Medicine·Kyle N KunzeJorge Chahla
Jul 1, 2020·Journal of Tissue Engineering and Regenerative Medicine·Sheila VeroneseAndrea Sbarbati
Jun 25, 2019·Frontiers in Cell and Developmental Biology·Letizia SenesiAntonio Gigante
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Aug 17, 2021·Stem Cells Translational Medicine·Angela Palumbo PiccionelloMichele Riccio

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Methods Mentioned

BETA
flow cytometry
PCR
PCRs

Software Mentioned

Rigenera
Excel Data Analysis
SPSS

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