Abstract
A new electrochemical approach to selective online measurements of dopamine (DA) release in the cerebral microdialysate is demonstrated with a non-oxidative mechanism based on the distinct reaction properties of DA and the excellent biocatalytic activity of laccase. To make the successful transition of the distinct sequential reaction properties of DA from a conceptual determination protocol to a practical online analytical system, laccase enzyme is immobilized onto magnetite nanoparticles and the nanoparticles are confined into a fused-silica capillary through an external magnetic field to fabricate a magnetic microreactor. The microreactor is placed in the upstream of the thin-layer electrochemical flow cell to efficiently catalyze the oxidation of DA into its quinonoid form and thereby initialize the sequential reactions including deprotonation, intramolecular cyclization, disproportionation and/or oxidation to finally give 5,6-dihydroxyindoline quinone. The electrochemical reduction of the produced 5,6-dihydroxyindoline quinone at bare glassy carbon electrode is used as the readout for the DA measurement. The laccase-immobilized microreactor is also found to catalyze the oxidation of ascorbic acid (AA) and 3,4-dihydroxyphen...Continue Reading
References
Jan 1, 1990·Journal of Neural Transmission. General Section·A BhardwajJ Weinberger
Jan 1, 1988·Journal of Neural Transmission·J Weinberger, J Nieves-Rosa
Mar 15, 1997·Biochimica Et Biophysica Acta·C FoppoliM A Rosei
Apr 20, 2001·Neuroscience·R E SuriM A Arbib
Apr 11, 2003·Nature·Paul E M PhillipsRegina M Carelli
Apr 6, 2006·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Morvarid KarimiJoel S Perlmutter
Citations
Jan 13, 2012·Accounts of Chemical Research·Meining ZhangLanqun Mao
Sep 19, 2012·Chemistry Central Journal·Yuanting LiDawei Li
Feb 27, 2016·Analytical Chemistry·Isabel Álvarez-Martos, Elena E Ferapontova
Sep 22, 2015·Analytica Chimica Acta·Yonghai SongLi Wang
Jul 19, 2016·Chemical Reviews·Mahmoud LabibShana O Kelley
Oct 25, 2016·Materials Science & Engineering. C, Materials for Biological Applications·Dongdong ZhangYanmin Zhang
Mar 19, 2014·Advanced Materials·Jingjing DengLanqun Mao
Dec 23, 2014·The Analyst·Yuqing LinLanqun Mao
May 13, 2020·Chemical Communications : Chem Comm·Wang ZhangYuqing Lin
Jan 1, 2016·3 Biotech·Pooja UpadhyayPavan Kumar Agrawal
Oct 9, 2020·Lab on a Chip·Jasper J A LozemanMathieu Odijk
Mar 19, 2013·Analytical Chemistry·Xulin LuLanqun Mao
Nov 10, 2010·Analytical Chemistry·Zipin ZhangLanqun Mao
Oct 5, 2013·Analytical Chemistry·Kun LiuLanqun Mao
Aug 13, 2013·Analytical Chemistry·Lei ZhangHuangxian Ju
Jan 24, 2012·Analytical Chemistry·Xuming ZhuangLanqun Mao
Jul 3, 2013·Analytical Chemistry·Wenjie MaLanqun Mao
Jul 10, 2013·Analytical Chemistry·Xia GaoLanqun Mao
May 8, 2020·Analytical Chemistry·Chao WangYuqing Lin
Mar 14, 2014·Analytical Chemistry·Yuqing LinLanqun Mao
Jan 21, 2017·Analytical Chemistry·Tongfang XiaoLanqun Mao
Feb 11, 2020·Analytical Chemistry·Yimin JiangHui Gu
Feb 15, 2020·Analytical Chemistry·Yongqi DingYuqing Lin
Sep 28, 2021·Microsystems & Nanoengineering·Changbo LiuXing Sheng
Nov 2, 2021·ACS Applied Materials & Interfaces·Guoyuan RenYuqing Lin