PMID: 9444996Jan 28, 1998Paper

A nonessential African swine fever virus gene UK is a significant virulence determinant in domestic swine

Journal of Virology
L ZsakD L Rock

Abstract

Sequence analysis of the right variable genomic region of the pathogenic African swine fever virus (ASFV) isolate E70 revealed a novel gene, UK, that is immediately upstream from the previously described ASFV virulence-associated gene NL-S (L. Zsak, Z. Lu, G. F. Kutish, J. G. Neilan, and D. L. Rock, J. Virol. 70:8865-8871, 1996). UK, transcriptionally oriented toward the right end of the genome, predicts a protein of 96 amino acids with a molecular mass of 10.7 kDa. Searches of genetic databases did not find significant similarity between UK and other known genes. Sequence analysis of the UK genes from several pathogenic ASFVs from Europe, the Caribbean, and Africa demonstrated that this gene was highly conserved among diverse pathogenic isolates, including those from both tick and pig sources. Polyclonal antibodies raised against the UK protein specifically precipitated a 15-kDa protein from ASFV-infected macrophage cell cultures as early as 2 h postinfection. A recombinant UK gene deletion mutant, deltaUK, and its revertant, UK-R, were constructed from the E70 isolate to study gene function. Although deletion of UK did not affect the growth characteristics of the virus in macrophage cell cultures, deltaUK exhibited reduced vi...Continue Reading

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Citations

Dec 26, 2008·Journal of Virology·Christopher L NethertonGeraldine Taylor
Sep 21, 2019·Transboundary and Emerging Diseases·Teshale TeklueHua-Ji Qiu
Apr 2, 2020·Journal of Veterinary Science·Dongwan YooHan Sang Yoo
Nov 20, 2019·Annual Review of Animal Biosciences·Linda K DixonDirk U Pfeiffer
Jul 7, 2020·Porcine Health Management·Laia Bosch-CamósFernando Rodriguez
Jul 31, 2019·The Journal of General Virology·Alexandra HübnerWalter Fuchs
Oct 11, 2017·Vaccines·Marisa AriasUNKNOWN Sanchez-Vizcaino
Mar 14, 2021·The Journal of Immunology : Official Journal of the American Association of Immunologists·Dan LiHaixue Zheng

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