A noninvasive method for distinguishing among canid species: amplification and enzyme restriction of DNA from dung

Molecular Ecology
E PaxinosR Fleischer

Abstract

Endangered San Joaquin kit foxes Vulpes macrotis mutica can be sympatrically distributed with as many as four other canids: red fox, gray fox, coyote and domestic dog. Canid scats are often found during routine fieldwork, but cannot be reliably identified to species. To detect and study the endangered kit fox, we developed mitochondrial DNA markers that can be amplified from small amounts of DNA extracted from scats. We amplified a 412-bp fragment of the mitochondrial cytochrome-b gene from scat samples and digested it with three restriction enzymes. The resulting restriction profiles discriminated among all five canid species and correctly identified 10 'unknown' fox scats to species in blind tests. We have applied our technique to identify canids species for an environmental management study and a conservation study. We envision that our protocol, and similar ones developed for other endangered species will be greatly used for conservation management in the future.

Citations

Nov 28, 2007·Experimental & Applied Acarology·Luís M RosalinoMargarida Santos-Reis
Oct 26, 2000·Molecular Ecology·L E FarrellM E Sunquist
Feb 12, 2011·Conservation Biology : the Journal of the Society for Conservation Biology·Sarah E Reed, Adina M Merenlender
Jun 27, 2014·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Iulia ZoicasInga D Neumann
Apr 26, 2002·Molecular Ecology·W O C Symondson
Jun 25, 2013·Mitochondrial DNA·Anthony CaragiuloGeorge Amato
Jul 20, 2005·The European Journal of Neuroscience·Lénaïc Monconduit, Luis Villanueva
May 1, 2009·Molecular Ecology Resources·Susana GonzálezJosé Maurício Barbanti Duarte
Mar 25, 2011·Molecular Ecology Resources·S RoquesJ A Godoy
Nov 27, 2010·Animal Science Journal = Nihon Chikusan Gakkaihō·Shinya IshiharaYukio Kanai
Apr 27, 2011·Molecular Ecology Resources·Jennifer R AdamsLisette P Waits
Nov 18, 2005·Analytical Biochemistry·Chris MurgatroydDietmar Spengler
May 7, 2015·Neuroscience Letters·Ataç SönmezMeryem Çalişir
Jul 17, 2004·Neuroreport·Anthony D Miller, Charles D Blaha
Sep 29, 2015·PloS One·Bibek YumnamYadvendradev V Jhala
Dec 2, 2020·Forensic Science International. Genetics·Sandeep Kumar GuptaSrinivas Yellapu
Mar 3, 2001·Science·D A SmithJ E Maldonado

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