A noninvasive reporter system to image adenoviral-mediated gene transfer to ovarian cancer xenografts

Gynecologic Oncology
T R ChaudhuriKurt Zinn

Abstract

Gene therapy trials for ovarian cancer would benefit from a noninvasive imaging modality to detect the location and extent of gene transfer. The human type 2 somatostatin receptor gene (hSSTr2) was evaluated as a reporter gene for imaging adenoviral (Ad) gene transfer to ovarian cancer. A replication-incompetent Ad vector encoding hSSTr2 (Ad-hSSTr2) was used to infect SKOV3.ip1 cells in vitro and tumors growing in nude mice. Gamma camera imaging detected uptake of 99m-Tc-P2045 (a somatostatin analogue) due to expressed hSSTr2. Specific uptake of 99m-Tc-P2045 was imaged in Ad-hSSTr2-infected cells in vitro. Noninvasive in vivo imaging detected gene transfer to intraperitoneal tumors. Uptake of 99m-Tc-P2045 (percentage dose per gram of tumor) averaged 2.2 and 0.18 for Ad-hSSTr2-injected mice and controls, respectively. This study reports the first noninvasive imaging method for imaging gene transfer to ovarian cancer. A human gene therapy trial is planned.

References

Jan 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·Y YamadaS Seino
Mar 1, 1995·Trends in Pharmacological Sciences·D HoyerJ E Taylor
Nov 18, 2000·Molecular Therapy : the Journal of the American Society of Gene Therapy·R D AlvarezD T Curiel
Apr 11, 2001·Nuclear Medicine and Biology·K R ZinnB E Rogers

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Citations

Nov 26, 2009·Molecular Therapy : the Journal of the American Society of Gene Therapy·Masato Yamamoto, David T Curiel
Aug 20, 2010·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·Ron ChenBuck E Rogers
Sep 14, 2004·Annals of Nuclear Medicine·Donald J BuchsbaumKurt R Zinn
Jul 23, 2004·Expert Opinion on Biological Therapy·Masato Yamamoto
Sep 28, 2010·Academic Radiology·Guangnan QuanHongyan Cheng
Oct 9, 2007·Nuclear Medicine and Biology·Inna SerganovaRonald Blasberg
Dec 7, 2006·Neuroimaging Clinics of North America·Dawid Schellingerhout
Oct 10, 2013·BioMed Research International·Ling WangHong Zhang
Nov 12, 2016·Cancer Gene Therapy·Z SaadatpourH Mirzaei
Sep 1, 2004·Molecular Therapy : the Journal of the American Society of Gene Therapy·J Andrea McCartDavid L Bartlett
Jan 29, 2003·Journal of Cellular Biochemistry. Supplement·R J Gillies
Sep 28, 2002·Journal of Magnetic Resonance Imaging : JMRI·Vijay SharmaDavid Piwnica-Worms
May 14, 2004·Gene Therapy·K ShahR Weissleder
Apr 27, 2001·Radiology·R Weissleder, U Mahmood
Apr 12, 2003·Technology in Cancer Research & Treatment·Tandra R ChaudhuriKurt R Zinn
Apr 25, 2020·Frontiers in Physiology·Madeleine Iafrate, Gilbert O Fruhwirth
Nov 9, 2018·Experimental and Therapeutic Medicine·Yanhua ZhaoYi Zhang
Dec 15, 2010·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·Hanwen ZhangPeter M Smith-Jones
Apr 4, 2020·Molecular Therapy : the Journal of the American Society of Gene Therapy·Candice Ashmore-HarrisGilbert O Fruhwirth

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