A noninvasive technique for the measurement of the energetic state of free-suspension mammalian cells

Biotechnology Progress
M Ben-TchavtchavadzeMario Jolicoeur

Abstract

A perfusion small-scale bioreactor allowing on-line monitoring of the cell energetic state was developed for free-suspension mammalian cells. The bioreactor was designed to perform in vivo nuclear magnetic resonance (NMR) spectroscopy, which is a noninvasive and nondestructive method that permits the monitoring of intracellular nutrient concentrations, metabolic precursors and intermediates, as well as metabolites and energy shuttles, such as ATP, ADP, and NADPH. The bioreactor was made of a 10-mm NMR tube following a fluidized bed design. Perfusion flow rate allowing for adequate oxygen supply was found to be above 0.79 mL min(-1) for high-density cell suspensions (10(8) cells). Chinese hamster ovary (CHO) cells were studied here as model system. Hydrodynamic studies using coloration/decoloration and residence time distribution measurements were realized to perfect bioreactor design as well as to determine operating conditions bestowing adequate homogeneous mixing and cell retention in the NMR reading zone. In vivo (31)P NMR was performed and demonstrated the small-scale bioreactor platform ability to monitor the cell physiological behavior for 30-min experiments.

References

Oct 1, 1976·Biotechnology and Bioengineering·C D Scott, C W Hancher
Dec 1, 1996·The American Journal of Physiology·R Steinlechner-MaranE Gnaiger
Feb 16, 2000·Journal of Biotechnology·S J HesseJ Visser
Apr 27, 2001·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·U PilatusZ M Bhujwalla
Nov 29, 2001·Biochemistry·Z Serber, V Dötsch
Dec 7, 2002·Biotechnology Progress·Laurence LamboursainMario Jolicoeur
May 3, 2003·Analytical and Bioanalytical Chemistry·Roland UlberSascha Beutel
Dec 8, 2004·Biotechnology and Bioengineering·Dorra GmatiMario Jolicoeur
Apr 19, 2005·Applied Microbiology and Biotechnology·Michael Butler
Dec 1, 2005·Biotechnology and Bioengineering·Jose R VallejosGovind Rao
May 27, 2006·Microbial Cell Factories·Jonathan I Betts, Frank Baganz
Sep 16, 2006·Biotechnology and Bioengineering·Fredrick O AbokaJoseph J Heijnen
Dec 20, 1992·Biotechnology and Bioengineering·A J MeehanA P Koretsky
May 1, 1994·Biotechnology and Bioengineering·S T SharfsteinD S Clark

❮ Previous
Next ❯

Citations

Apr 12, 2016·Frontiers in Molecular Biosciences·Michel PrudentNiels Lion

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.