A nonoxytocinergic prolactin releasing factor and a nondopaminergic prolactin inhibiting factor in bovine neurointermediate lobe extracts: in vitro and in vivo studies

Endocrinology
W K SamsonR J Fulton

Abstract

Several peptidergic PRL-releasing factors (PRFs) have been described; however, none have been proven to be of primary physiological importance in the control of hormone release. Similarly dopamine withdrawal alone cannot completely explain the profiles of PRL secretion observed under a variety of conditions. We describe here the isolation in semipurified form of both a PRF and a PRL-inhibiting factor (PIF) from bovine neurointermediate lobe (NIL) extracts. Acid extracts of bovine NILs stimulated, in a dose-related manner, PRL release from cultured anterior pituitary cells, even after immunoabsorption of endogenous oxytocin from the extract. PIF and PRF activities were semipurified from NIL extracts by Sephadex chromatography and detected by in vitro and in vitro bioassays. The PRF material could be separated from oxytocin by gel sieving and was active in the presence of dopamine in vitro unlike synthetic oxytocin and in cell preparations in which the oxytocin-responsive lactotrophs had been removed by selective cytotoxin cell targeting using an oxytocin-ricin A chain cytotoxic conjugate. The PRF material stimulated PRL secretion in a dose-dependent fashion in conscious male rats after iv injection. The PIF material comigrated o...Continue Reading

Citations

Sep 1, 1992·Molecular and Cellular Endocrinology·R J Mogg, F R Boockfor
Nov 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·R E BlackburnJ G Verbalis
Oct 1, 1992·Journal of Neuroendocrinology·F L HuangW K Samson
Jun 1, 1994·Journal of Neuroendocrinology·J DohanicsJ G Verbalis
Sep 12, 2012·Journal of Neuroendocrinology·G L C YostenW K Samson
Sep 16, 1992·Biochemical and Biophysical Research Communications·W K Samson
Jun 15, 1990·Biochemical and Biophysical Research Communications·W K SamsonF L Huang
Jun 24, 2015·The Journal of Endocrinology·David R Grattan
Jun 1, 1992·Molecular and Cellular Endocrinology·J Schwartz
Oct 4, 2000·Physiological Reviews·M E FreemanG Nagy
Nov 28, 2001·Journal of Neuroendocrinology·B E TóthG M Nagy

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