PMID: 11607083Jun 1, 1990Paper

A nontransformable Triticum monococcum monocotyledonous culture produces the potent Agrobacterium vir-inducing compound ethyl ferulate

Proceedings of the National Academy of Sciences of the United States of America
E MessensS E Stachel

Abstract

Exudates of dicotyledonous plants contain specific phenolic signal molecules, such as acetosyringone, which serve as potent inducers for the expression of the virulence (vir) regulon of the phytopathogen Agrobacterium tumefaciens. This induction activates the Agrobacterium T-DNA transfer process to initiate the genetic transformation of target plant cells. Wounded and metabolically active plant cells are particularly susceptible to Agrobacterium infection, and these cells specifically produce vir-inducing molecules. Most monocotyledonous, as opposed to dicotyledonous, species are resistant to Agrobacterium transformation. One hypothesis for this resistance is that nonsusceptible monocotyledonous cells fail to produce vir signal molecules and, thus, are not recognized by Agrobacterium as transformation targets. Here we demonstrate that monocotyledonous cells make such molecules, and, furthermore, we purify the inducer produced by a Triticum monococcum suspension culture that is resistant to Agrobacterium infection. This molecule is shown to correspond to ethyl ferulate [C12H14O4; 3-(4-hydroxy-3-methoxyphenyl)-2-propenoic acid ethyl ester], to be more active for vir induction at low concentrations than acetosyringone, and to be p...Continue Reading

References

Oct 27, 1978·Biochimica Et Biophysica Acta·A C Braun
Jan 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·S E StachelP C Zambryski
Aug 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·B BytebierJ P Hernalsteens
Jun 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·S UsamiY Machida

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Citations

May 1, 1992·Plant Molecular Biology·P J Hooykaas, R A Schilperoort
Oct 1, 1995·Plant Molecular Biology·K VijayachandraK Veluthambi
Jul 1, 1996·World Journal of Microbiology & Biotechnology·K Weising, G Kahl
Dec 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·E JarchowB Hohn
Mar 12, 2004·Annual Review of Plant Physiology and Plant Molecular Biology·Stanton B. Gelvin
Aug 9, 2020·Frontiers in Plant Science·Stephanie LiuB Daniel Pierce
Mar 1, 1992·Microbiological Reviews·S C Winans

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