PMID: 6413476Aug 1, 1983Paper

A note on inoculum reproducibility: a comparison between solid and liquid culture

The Journal of Applied Bacteriology
M M Al-Hiti, Peter Gilbert

Abstract

The level of reproducibility for replicate determinations of drug sensitivity was significantly greater for liquid than for solid cultures and decreased markedly with the density of colonies upon seeded agar plates. Inocula of Escherichia coli and Pseudomonas aeruginosa were significantly less sensitive towards chlorhexidine when derived from liquid rather than solid culture. We therefore suggest that only liquid cultures be used for the preparation of challenge inocula for regulatory tests of antimicrobial activity.

References

May 1, 1977·The Journal of Antimicrobial Chemotherapy·M R Brown
Dec 1, 1969·Journal of General Microbiology·M R Brown, J Melling
Dec 1, 1968·Journal of General Microbiology·M R Brown, J Melling
Aug 1, 1980·The Journal of Applied Bacteriology·M M Al-Hiti, P Gilbert

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Citations

Jan 1, 1993·The Journal of Applied Bacteriology·M R Brown, P Gilbert
Oct 19, 2011·Antimicrobial Agents and Chemotherapy·Regina Helena PiresMaria Jose Soares Mendes-Giannini
Dec 24, 2005·International Journal of Hygiene and Environmental Health·Florian BrillWolfgang Sand
Apr 27, 1999·The Journal of Pharmacy and Pharmacology·G W HanlonS P Denyer
Apr 1, 1989·Journal of Dairy Science·J S Hogan, K L Smith
May 1, 1989·Applied and Environmental Microbiology·P GilbertM R Brown

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