A novel 2,5-bis(benzo[d]thiazol-2-yl)phenol scaffold-based ratiometric fluorescent probe for sensing cysteine in aqueous solution and serum

Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
Meng LiNuonuo Zhang

Abstract

An efficient and novel 2,5-bis(benzo[d]thiazol-2-yl)phenol scaffold-based ratiometric fluorescent probe BTP-Cys for the sensing of cysteine has been developed. The probe BTP-Cys with acrylates moiety, as recognition site, has been successfully constructed on account of the excited state intramolecular proton transfer (ESIPT) mechanism. Upon the treatment with Cys (0-250 μM), this probe BTP-Cys exhibits a dramatic fluorescent intensity ratios enhancement (from 0.03 to 18.3) and a large emission shift (113 nm). The detection limit of this probe is as low as 3.8 × 10-7 M. Importantly, the concentration and time dependent of Cys in bovine serum albumin (BSA) has also been measured, indicating that BTP-Cys could be a biocompatible and rapid probe for Cys in vitro.

Related Concepts

Metazoa
Bos indicus
Cysteine Hydrochloride
Fluorochromes
Hydrogen-Ion Concentration
Phenols
Serum Albumin, Bovine
Fluorescence Spectroscopy
Molecular Probe Techniques
Limit of Detection

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