A novel accurate LC-MS/MS method for quantitative determination of Z-lumirubin

Scientific Reports
Jana JašprováLibor Vítek

Abstract

Although phototherapy (PT) is a standard treatment for neonatal jaundice, no validated clinical methods for determination of bilirubin phototherapy products are available. Thus, the aim of our study was to establish a such method for clinical use. To achieve this aim, a LC-MS/MS assay for simultaneous determination of Z-lumirubin (LR) and unconjugated bilirubin (UCB) was conducted. LR was purified after irradiation of UCB at 460 nm. The assay was tested on human sera from PT-treated neonates. Samples were separated on a HPLC system with a triple quadrupole mass spectrometer detector. The instrument response was linear up to 5.8 and 23.4 mg/dL for LR and UCB, respectively, with submicromolar limits of detection and validity parameters relevant for use in clinical medicine. Exposure of newborns to PT raised serum LR concentrations three-fold (p < 0.01), but the absolute concentrations were low (0.37 ± 0.16 mg/dL), despite a dramatic decrease of serum UCB concentrations (13.6 ± 2.2 vs. 10.3 ± 3.3 mg/dL, p < 0.01). A LC-MS/MS method for the simultaneous determination of LR and UCB in human serum was established and validated for clinical use. This method should help to monitor neonates on PT, as well as to improve our understanding...Continue Reading

References

Oct 1, 1979·The Biochemical Journal·M S StollJ E Zarembo
Mar 1, 1990·American Journal of Diseases of Children·T B NewmanD K Stevenson
Jan 1, 1985·Annals of the New York Academy of Sciences·A F McDonagh
Sep 1, 1972·The Biochemical Journal·A F McDonagh, F Assisi
May 1, 1984·The Biochemical Journal·R BonnettM S Stoll
Jan 1, 1982·The Biochemical Journal·M S StollR Bonnett
May 24, 1958·Lancet·R J CREMERD H RICHARDS
Jul 3, 2004·Pediatrics·UNKNOWN American Academy of Pediatrics Subcommittee on Hyperbilirubinemia
Feb 3, 2005·Seminars in Perinatology·Hendrik J VremanDavid K Stevenson
Feb 29, 2008·The New England Journal of Medicine·M Jeffrey Maisels, Antony F McDonagh
Apr 1, 2008·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Jaroslav ZelenkaLibor Vítek
Oct 31, 2008·The New England Journal of Medicine·Brenda H MorrisUNKNOWN NICHD Neonatal Research Network
Dec 17, 2009·Pediatrics·Sotirios FouzasAnastasia Varvarigou
Jun 2, 2012·Journal of Perinatology : Official Journal of the California Perinatal Association·J E TysonUNKNOWN Eunice Kennedy Shriver National Institute of Child Health and Human Development Neonatal Research Network
Nov 20, 2015·Journal of Perinatology : Official Journal of the California Perinatal Association·N RamyH Aly
Feb 2, 2016·PloS One·Jana JasprovaLibor Vitek
May 12, 2018·Scientific Reports·J JašprováL Vítek
Jan 27, 2019·International Journal of Cancer. Journal International Du Cancer·Nathalie AugerThuy Mai Luu

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Citations

Aug 28, 2020·Circulation Journal : Official Journal of the Japanese Circulation Society·Lenka EremiasovaLibor Vitek
Nov 5, 2020·International Journal of Molecular Sciences·Annalisa BiancoCristina Bellarosa
Mar 23, 2021·Frontiers in Pharmacology·Aleš DvořákLibor Vítek
Oct 3, 2020·The Journal of Organic Chemistry·Dominik MadeaPetr Klán

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