A novel affordable reagent for room temperature storage and transport of fecal samples for metagenomic analyses

Microbiome
Mo HanJunhua Li

Abstract

The number of large-scale studies on the gut microbiota in human cohorts is rapidly increasing. However, the few and expensive options for storage of fecal samples at room temperature have been an obstacle for large-scale metagenomic studies and the development of clinical/commercial personal metagenomic sequencing. In this study, we systematically tested a novel N-octylpyridinium bromide-based fecal sample preservation method and compared it with other currently used storage methods. We found that the N-octylpyridinium bromide-based method enabled preservation of the bacterial composition in fecal samples transported and stored at room temperature for up to at least 14 days. We describe a novel chemical stabilizer that allows cost-effective transportation and storage at room temperature for several days with preservation of bacterial composition. This method will facilitate sample collection even in remote area and also enable transport via normal commercial transportation routes.

References

Jul 14, 2011·Nature Reviews. Neuroscience·Emeran A Mayer
Sep 14, 2012·Nature·Valentina Tremaroli, Fredrik Bäckhed
Sep 7, 2013·Science·Alan W Walker, Julian Parkhill
Jul 7, 2014·Nature Biotechnology·Junhua LiUNKNOWN MetaHIT Consortium
Apr 17, 2015·Biopreservation and Biobanking·Conny MathayFay Betsou
Jan 23, 2016·Applied Microbiology and Biotechnology·Qing HeLiang Xiao
Jan 5, 2019·Physical Review Letters·D AdeyUNKNOWN Daya Bay Collaboration

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Citations

Jul 21, 2020·BioMed Research International·Zhiming LiYuanxiang Gao
Jun 18, 2020·Bioinformatics and Biology Insights·Zonghui PengKarsten Kristiansen
Mar 10, 2021·Maternal Health, Neonatology and Perinatology·Jacob A F WestawayDonna Rudd
Jul 3, 2021·Nutrients·Sergio Pérez-BurilloJosé Ángel Rufián-Henares
Jun 13, 2021·Genomics, Proteomics & Bioinformatics·Zhuye JieHuijue Jia
Aug 16, 2021·Journal of Genetics and Genomics = Yi Chuan Xue Bao·Chen ChenHuijue Jia

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Methods Mentioned

BETA
PCR
amplicon sequencing

Software Mentioned

PERMANOVA
SOAPaligner2
vegan
ggplot2
R
FASTX Toolkit

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