A novel allele of ASY3 is associated with greater meiotic stability in autotetraploid Arabidopsis lyrata

PLoS Genetics
Paul J SeearJ D Higgins

Abstract

In this study we performed a genotype-phenotype association analysis of meiotic stability in 10 autotetraploid Arabidopsis lyrata and A. lyrata/A. arenosa hybrid populations collected from the Wachau region and East Austrian Forealps. The aim was to determine the effect of eight meiosis genes under extreme selection upon adaptation to whole genome duplication. Individual plants were genotyped by high-throughput sequencing of the eight meiosis genes (ASY1, ASY3, PDS5b, PRD3, REC8, SMC3, ZYP1a/b) implicated in synaptonemal complex formation and phenotyped by assessing meiotic metaphase I chromosome configurations. Our results reveal that meiotic stability varied greatly (20-100%) between individual tetraploid plants and associated with segregation of a novel ASYNAPSIS3 (ASY3) allele derived from A. lyrata. The ASY3 allele that associates with meiotic stability possesses a putative in-frame tandem duplication (TD) of a serine-rich region upstream of the coiled-coil domain that appears to have arisen at sites of DNA microhomology. The frequency of multivalents observed in plants homozygous for the ASY3 TD haplotype was significantly lower than in plants heterozygous for ASY3 TD/ND (non-duplicated) haplotypes. The chiasma distributi...Continue Reading

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Citations

Jan 18, 2021·The New Phytologist·Roswitha Schmickl, Levi Yant
Mar 31, 2021·Molecular Biology and Evolution·Magdalena BohutínskáLevi Yant

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Methods Mentioned

BETA
genotyping
PCR

Software Mentioned

MrBayes
Geneious
Ensembl
BLASTN
NIS Elements
NetPhos3
PhyML
Quality Trimming
MiSeq
BBDuk Adaptor /

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