A novel amperometric biosensor based on gold nanoparticles anchored on reduced graphene oxide for sensitive detection of l-lactate tumor biomarker

Biosensors & Bioelectronics
Sawsen AzzouziCamelia Bala

Abstract

In this work, a novel amperometric biosensor based on gold nanoparticles anchored on reduced graphene oxide (RGO-AuNPs) and l-lactate dehydrogenase (LDH) was developed for the sensing of l-lactate. Firstly, the RGO-AuNPs modified screen printed electrodes were tested for NADH detection showing a wide dynamic range and a low detection limit. Next, the biosensor was constructed by incorporating both enzyme and RGO-AuNPs in a sol gel matrix derived from tetrametoxysilane and methyltrimetoxysilane. The enzyme loading, working pH, and coenzyme concentration were optimized. The biosensor linearly responded to l-lactate in the range of 10µM-5mM and showed a good specific sensitivity of 154µA/mMcm(2) with a detection limit of 0.13µM. This was accompanied by good reproducibility and operational stability. Tests on artificial serum proved that l-lactate can be determined practically without interferences from commonly interfering compounds such as urate, paracetamol and l-ascorbate. Our LDH/RGO-AuNPs/SPCE based biosensor thus performs as electrochemical device for the detection of l-lactate as a viable early cancer bio-marker.

Citations

Aug 15, 2018·Sensors·Gerard CumminsMarc P Y Desmulliez
Jun 28, 2019·Frontiers in Chemistry·Haizhou HuangLitao Sun
Dec 7, 2016·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Ali OthmanSilvana Andreescu
Jun 7, 2015·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Kostiantyn TurcheniukSabine Szunerits
May 15, 2018·Frontiers in Bioengineering and Biotechnology·Matthew S BrownAhyeon Koh
Jul 14, 2016·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Bhaskar Manna, C Retna Raj

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