A novel analgesic approach to optogenetically and specifically inhibit pain transmission using TRPV1 promoter

Brain Research
Bei LiLi-Yang Chiang

Abstract

Chronic pain is a pathological condition that results in significant loss of life quality, but so far no specific treatment for chronic pain has been developed. Currently available analgesia drugs are either not specific enough or have severe side effects. Therefore a non-invasive approach with high specificity to inhibit nociception becomes essential. In this study, a recombinant virus (AAV5-TRPV1-ArchT-eGFP) was constructed and injected into the mouse dorsal root ganglion (DRG). The Transient Receptor Potential Vanilloid type 1 (TRPV1) channel promoter was used to selectively express inhibitory light-sensitive pump ArchT (the archaerhodopsin from Halorubrum strain TP009) in nociceptive DRG neurons. The successful transfer of ArchT gene was confirmed by a robust expression of green florescent protein in the DRG neurons. In vivo behavioral tests demonstrated that both the mechanical paw withdrawal threshold and the radiant heat evoked paw withdrawal latency were significantly increased upon illumination by a 532 nm green laser light to the paw of a viral-vector injected mice, while the same laser light did not induce any observable change in naïve mice. In conclusion, we have established a novel analgesic approach that can noni...Continue Reading

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Citations

May 6, 2016·Science Translational Medicine·Kate L MontgomeryScott L Delp
Oct 27, 2016·Neuroscience·Feng WangYves De Koninck
Aug 4, 2016·Scientific Reports·Shrivats M IyerScott L Delp
Jul 21, 2017·Pain Management·Hélène BeaudryPhilippe Séguéla
Jun 22, 2017·British Journal of Pharmacology·Alexandre MourotRichard H Kramer
Dec 1, 2017·Nature Neuroscience·Erica RodriguezFan Wang
Aug 23, 2019·Advanced Materials·Angelo Homayoun AllXiaogang Liu
Jan 4, 2019·Nature·Aaron D MickleJohn A Rogers
Feb 20, 2020·Critical Reviews in Biochemistry and Molecular Biology·Xiang XuXiaofeng Jia
Jun 4, 2019·Pain Medicine : the Official Journal of the American Academy of Pain Medicine·Michael A FishmanRobert Levy
Jul 1, 2020·Frontiers in Neural Circuits·Erika K HardingRobert P Bonin
Aug 17, 2018·Pain·Aaron D Mickle, Robert W Gereau

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