A novel analysis method for diterpenoids in rat plasma by liquid chromatography-electrospray ionization mass spectrometry

Analytical Biochemistry
Yingfeng DuLantong Zhang

Abstract

A sensitive, specific, and rapid liquid chromatography-mass spectrometry (LC-MS) method was developed and validated for analysis of lasiodonin, oridonin, ponicidin, and rabdoternin A in rat plasma using sulfamethoxazole as an internal standard (IS). The plasma samples were pretreated and extracted by liquid-liquid extraction. Chromatographic separation was performed on a C(18) column with linear gradient elution using water and methanol, both of which were acidified with 0.1% aqueous formic acid, at a flow rate of 0.8 ml/min. Detection was accomplished by scanning with multiple reaction monitoring (MRM) via an electrospray ionization (ESI) source. Higher sensitivity was achieved by setting three scanning periods in a novel detection mode. The optimized mass transition ion pairs (m/z) for quantitation were 365.3/347.3 for lasiodonin and oridonin, 361.2/343.2 for ponicidin, 363.2/283.1 for rabdoternin A, and 254.1/156.0 for IS. The total run time was 13.50 min between injections. The specificity, linearity, accuracy, precision, recovery, matrix effect, and several stabilities were validated for all analytes in the rat plasma samples. In conclusion, the validation results demonstrate that this method is robust and specific. The pr...Continue Reading

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Citations

Dec 18, 2013·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Xuran ZhiLantong Zhang
Nov 21, 2015·Journal of Traditional and Complementary Medicine·Piyush MehtaK R Mahadik
Aug 21, 2015·Drugs in R&D·Brice Ayissi Owona, Herman J Schluesener
Feb 8, 2011·The International Journal of Biochemistry & Cell Biology·Chun-yang LiJin-ku Bao
Aug 27, 2016·International Journal of Molecular Sciences·Dahong LiJinyi Xu
Aug 30, 2011·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Jingcheng TangShiqi Peng
Jun 30, 2019·ACS Applied Materials & Interfaces·Dongdong ChaiHong Jiang

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