A novel and sensitive fluorescence immunoassay for the detection of fluoroquinolones in animal-derived foods using upconversion nanoparticles as labels

Analytical and Bioanalytical Chemistry
Gaoshuang HuShuo Wang

Abstract

A novel fluorescence immunoassay to detect fluoroquinolones in animal-derived foods was developed for the first time by use of upconversion nanoparticles as signal-probe labels. The bioassay system was established by the use of coating-antigen-modified polystyrene particles as immune-sensing probes for separation and anti-norfloxacin monoclonal antibody conjugated with carboxyl-functionalized NaYF4:Yb,Er upconversion nanoparticles which were prepared via a pyrolysis method and a subsequent ligand exchange process as fluorescent-signal probes (emission intensity recorded at 542 nm with excitation at 980 nm). Under optimized conditions, detection of fluoroquinolones was performed easily. The detection limit of this fluorescence immunoassay for norfloxacin, for example, was 10 pg mL(-1), within a wide linear range of 10 pg mL(-1) to 10 ng mL(-1) (R (2)  = 0.9959). For specificity analysis, the data obtained indicate this method could be applied in broad-spectrum detection of fluoroquinolones. The recoveries of norfloxacin-spiked animal-derived foods ranged from 82.37 to 132.22 %, with coefficients of variation of 0.24-25.06 %. The extraction procedure was rapid and simple, especially for milk samples, which could be analyzed direc...Continue Reading

References

Jul 4, 2007·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Ching-Ling ChengChen-Hsi Chou
Jun 19, 2012·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Qinghai XiaMousheng Liu
Apr 17, 2013·Biosensors & Bioelectronics·Bharati AgrawalYoon-Bo Shim
May 9, 2015·Materials Science & Engineering. C, Materials for Biological Applications·Herman S MansurMaria de Fatima Leite

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Citations

Apr 15, 2016·Critical Reviews in Analytical Chemistry·Yang LuShuo Wang
Jul 11, 2019·Analytical and Bioanalytical Chemistry·Qiuju ZhouKejun Tan
Mar 20, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ana-Mădălina MăciucăValentina Uivarosi
Nov 19, 2020·The Analyst·Riikka PeltomaaMaría C Moreno-Bondi
Feb 20, 2019·Trends in Biotechnology·Marjan MajdinasabJean Louis Marty
Jun 24, 2021·Critical Reviews in Food Science and Nutrition·Deshmukh Abdul HakeemHongli Wen

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