A novel animal model of severe pancreatitis in mice and its differences to the rat

Surgery
Werner HartwigJens Werner

Abstract

A noninvasive model of necrohemorrhagic pancreatitis induced by simultaneous intravenous cerulein/enterokinase (EK) infusion has recently been established in rats. The aim of the present study was to establish this new model in mice and to compare it with the rat model. Male Balb/C mice (20 to 25 g) were used for the experiments. Pancreatitis was induced by simultaneous intravenous infusion of cerulein and EK. Controls were infused with either 0.9% NaCl, cerulein, or EK. Animals were humanely killed 6 hours after start of infusions. Pancreatic and pulmonary injury was assessed by histology, wet-to-dry weight ratio, and myeloperoxidase activity. Systemic cytokine, amylase, and lactate dehydrogenase (LDH) levels in blood were measured to assess pancreatic and systemic inflammatory response. To evaluate the role of protease activity in this model, trypsin, cathepsin B, and elastase activity were measured in pancreatic tissue. Survival experiments were performed to determine survival time and tissue injury in the later course of the disease. Mice with simultaneous cerulein/EK infusion developed marked local and systemic organ injury compared with those animals who received cerulein or EK alone. Pancreatic and pulmonary injury incre...Continue Reading

References

Mar 11, 1977·Virchows Archiv. A, Pathological Anatomy and Histology·M Lampel, H F Kern
Jan 1, 1992·European Surgical Research. Europäische Chirurgische Forschung. Recherches Chirurgicales Européennes·C NiederauL D Ferrell
Jan 1, 1992·Annals of Surgery·J SchmidtA L Warshaw
Oct 1, 1989·The Journal of Clinical Investigation·A SalujaM Steer
Nov 1, 1982·Digestive Diseases and Sciences·G AdlerH F Kern
Jun 24, 2000·European Journal of Biochemistry·K I HulkowerB F Sloane
Aug 23, 2000·International Journal of Colorectal Disease·T FoitzikH J Buhr
Jan 13, 2004·American Journal of Physiology. Gastrointestinal and Liver Physiology·Madhav Bhatia
Aug 17, 2004·American Journal of Physiology. Gastrointestinal and Liver Physiology·Werner HartwigMarkus W Büchler
Nov 9, 2004·Current Opinion in Cell Biology·Aimee L Edinger, Craig B Thompson
Nov 17, 2004·British Journal of Cancer·R D BrammerM C Eggo
Dec 13, 2005·The Journal of Biological Chemistry·Olga A MareninovaAnna S Gukovskaya
Dec 16, 2006·American Journal of Physiology. Gastrointestinal and Liver Physiology·Rajinder DawraAshok K Saluja
Jan 2, 2007·Pancreas·Yuk Cheung Chan, Po Sing Leung
Mar 12, 2008·HPB : the Official Journal of the International Hepato Pancreato Biliary Association·Kim Hue SuChristopher Christophi

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Citations

Oct 17, 2015·International Journal of Surgery·Serkan ArslanUnal Uluca
Jun 16, 2016·Scientific Reports·Yunan WangGeorge Liu
Jun 12, 2019·International Journal of Molecular Sciences·Pedro Silva-VazJosé Guilherme Tralhão
Sep 27, 2014·Medical Science Monitor : International Medical Journal of Experimental and Clinical Research·Bin ZhuYong Jiang

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