PMID: 9167941Apr 1, 1997Paper

A novel animal model to evaluate oxygen derived free radical damage in soft tissue

Free Radical Research
L van der LaanR J Goris

Abstract

We present a novel animal model which allows the continuous intra-arterial infusion in one hindlimb of non-anaesthetized rats, without inducing ischemia. Using this model the effect of continuous infusion (1 ml/h) for 24 h with tert-butylhydroperoxide (tert-BuOOH) at a concentration of 25 mM on soft tissue of the left hind limb was studied and compared to the effect of saline infusion (control group). The tert-BuOOH-infused foot showed increased skin temperature, increased circumference, redness of the plantar skin, impaired function and increased pain sensation, while in the contralateral foot and in rats only perfused with saline these signs of inflammation were absent (p < 0.01). Histological analysis of the left gastrocnemius muscle showed edema, muscle cell degeneration with a patchy distribution pattern and vascular damage. All these features increased in severity from 4 to 24 h tert-BuOOH infusion. After 24 h of tert-BuOOH infusion infiltration of neutrophils in the interstitium was observed. Vascular permeability, expressed as left to right gastrocnemius muscle 99mTc-IgG uptake ratio, was similarly increased after 4 h (2.09 +/- 0.26) and 12 h (2.04 +/- 0.08) of tert-BuOOH infusion compared to saline (1.05 +/- 0.08) (p <...Continue Reading

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Citations

Mar 12, 2004·Journal of Ethnopharmacology·Ilhami GülçinMehmet Emin Büyükokuroglu
Dec 21, 2004·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Klaus-D SchaserThomas Mittlmeier
Apr 11, 2003·Journal of Ethnopharmacology·Ilhami GülçinO Irfan Küfrevioglu
Jun 15, 2001·ILAR Journal·Ke Ren, Ronald Dubner
Sep 19, 2012·The Journal of Foot and Ankle Surgery : Official Publication of the American College of Foot and Ankle Surgeons·Naohiro ShibuyaDaniel C Jupiter
Oct 21, 2009·Foot and Ankle Surgery : Official Journal of the European Society of Foot and Ankle Surgeons·Jean-Luc BesseBernard Moyen
Aug 19, 2008·The Journal of Surgical Research·Edward C T H TanR Jan A Goris
Sep 25, 2008·Phytotherapy Research : PTR·Samra Bashir, Anwar H Gilani
Mar 28, 2008·The European Journal of Neuroscience·Yosuke SawadaShigeo Kobayashi
Jan 23, 2013·Pain Practice : the Official Journal of World Institute of Pain·Adriana A MiclescuTorsten Gordh
Mar 1, 2014·International Journal of Rheumatic Diseases·Tuba BaykalKazim Senel
Jul 15, 2015·Oxidative Medicine and Cellular Longevity·Suryamin LimanMichael Irwin
Nov 5, 1997·The Journal of Surgical Research·L van der LaanR J Goris
Dec 10, 2014·European Journal of Orthopaedic Surgery & Traumatology : Orthopédie Traumatologie·Sanjay MeenaBuddhadev Chowdhury
Jun 10, 2008·Pain·Elon EisenbergRafael M Nagler
Aug 11, 2015·Journal of Advanced Research·Ângelo de FátimaLuzia V Modolo
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Sep 25, 2002·Physiological Reviews·Linda R Watkins, Steven F Maier
May 2, 2018·The Journal of Pain : Official Journal of the American Pain Society·Tian-Zhi GuoJohn David Clark

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