A novel anti-platelet monoclonal antibody induces mouse platelet aggregation through an Fc receptor-independent mechanism

Biochemical and Biophysical Research Communications
Y KatoT Tsuruo

Abstract

Platelets are nonproliferative and terminally differentiated cells. Platelets offer an attractive model system to study the various biochemical events leading to structural and functional alterations in activated cells. When platelets are exposed to stimuli, they are activated, undergo a dramatic shape change, adhere to each other, and aggregate. Several monoclonal antibodies (mAbs) that recognize CD9, GPIIb/IIIa (alpha IIb beta 3 intergrins), or GPIV are known to stimulate human platelet aggregation. However, no mAbs able to induce aggregation of mouse platelets have been reported. We have established an anti-mouse platelet mAb (AIP21) that can promote mouse platelet aggregation by itself. Because mouse platelets did not express the Fc receptor (FcR, CD32) on their surfaces and because AIP21 is an IgM subclass, AIP21 might promote platelet aggregation through an FcR-independent mechanism. We could not identify the antigen recognized by AIP21, but flow cytometric analysis revealed that it was not identical to CD9, GPIV, or integrins (i.e., alpha IIb, alpha v, alpha 5, alpha 6, beta 1, and beta 3 integrins). During the aggregation of mouse platelets mediated by AIP21, several 50-68-kDa proteins are rapidly phosphorylated at tyro...Continue Reading

Citations

Jul 24, 2010·The American Journal of Pathology·Sapna DeviMichael J Hickey
Mar 6, 2003·Vox Sanguinis·Michael P Reilly, Steven E McKenzie
Aug 13, 2002·Current Opinion in Hematology·Michael P Reilly, Steven E McKenzie

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