A Novel Antimicrobial Coating Represses Biofilm and Virulence-Related Genes in Methicillin-Resistant Staphylococcus aureus

Frontiers in Microbiology
Ankita VaishampayanElisabeth Grohmann

Abstract

Methicillin-resistant Staphylococcus aureus (MRSA) has become an important cause of hospital-acquired infections worldwide. It is one of the most threatening pathogens due to its multi-drug resistance and strong biofilm-forming capacity. Thus, there is an urgent need for novel alternative strategies to combat bacterial infections. Recently, we demonstrated that a novel antimicrobial surface coating, AGXX®, consisting of micro-galvanic elements of the two noble metals, silver and ruthenium, surface-conditioned with ascorbic acid, efficiently inhibits MRSA growth. In this study, we demonstrated that the antimicrobial coating caused a significant reduction in biofilm formation (46%) of the clinical MRSA isolate, S. aureus 04-02981. To understand the molecular mechanism of the antimicrobial coating, we exposed S. aureus 04-02981 for different time-periods to the coating and investigated its molecular response via next-generation RNA-sequencing. A conventional antimicrobial silver coating served as a control. RNA-sequencing demonstrated down-regulation of many biofilm-associated genes and of genes related to virulence of S. aureus. The antimicrobial substance also down-regulated the two-component quorum-sensing system agr suggesting...Continue Reading

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Citations

Apr 6, 2019·Biofouling·Fernanda Teresinha de Almeida Sayão de EmeriJanaina de Cassia Orlandi Sardi
Apr 10, 2019·Current Medicinal Chemistry·Iolanda Francolini, Antonella Piozzi
Feb 6, 2021·Biological Chemistry·Nico LinznerHaike Antelmann
Jan 9, 2020·Microbial Pathogenesis·Sharon Vieira Dos ReisAlexandre José Macedo

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Datasets Mentioned

BETA
GSE103064

Methods Mentioned

BETA
RNA-Seq
PCR
confocal microscopy

Software Mentioned

Fiji ( ImageJ
Cufflinks
REx
GSEA
SAMtools
Pro
IonProton
Bowtie2
LightCycler ® 480
SigmaPlot

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