A novel antitumor compound, NC-190, induces topoisomerase II-dependent DNA cleavage and DNA fragmentation

Cancer Chemotherapy and Pharmacology
T YamagishiS Otomo

Abstract

A novel benzophenazine derivative, NC-190, is a potent antitumor compound. NC-190 has been shown to inhibit the DNA strand-passing activity of DNA topoisomerase II. We investigated further the mode of action of NC-190 against DNA topoisomerase II and DNA fragmentation. NC-190 inhibited the decatenation activity of purified topoisomerase II, but had only a weak inhibitory effect against topoisomerase I. A topoisomerase II-dependent DNA cleavage assay showed that NC-190 inhibited the enzyme activity by stabilizing a topoisomerase II-DNA cleavable complex. NC-190 induced growth inhibition, protein-linked DNA breaks, and DNA fragmentation in cultured HL-60 cells in a dose-dependent manner. These activities of NC-190 in HL-60 cells were comparable to those of etoposide (VP-16). These results demonstrate a good correlation among growth inhibition, topoisomerase II-dependent DNA cleavage, and DNA fragmentation induced by NC-190. A DNA unwinding assay showed that NC-190 had intercalating activity, but its activity appeared to be weaker than those of ethidium bromide and adriamycin. These results indicate that the mechanism by which NC-190 exhibits antitumor activity may be the inhibition of topoisomerase II.

Citations

Feb 18, 2012·Natural Product Reports·A CimminoR Kiss
Aug 13, 2013·Evidence-based Complementary and Alternative Medicine : ECAM·Tung-Ting ShamShun-Wan Chan
Mar 11, 2004·Chemical Reviews·Jane Buus Laursen, John Nielsen
Dec 24, 2004·Journal of Medicinal Chemistry·Serge Christmann-FranckSerge Fermandjian

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