A Novel AP2/ERF Transcription Factor, OsRPH1, Negatively Regulates Plant Height in Rice

Frontiers in Plant Science
Ziming MaWenzhu Jiang

Abstract

The APETALA 2/ethylene response factors (AP2/ERF) are widespread in the plant kingdom and play essential roles in regulating plant growth and development as well as defense responses. In this study, a novel rice AP2/ERF transcription factor gene, OsRPH1, was isolated and functionally characterized. OsRPH1 falls into group-IVa of the AP2/ERF family. OsRPH1 protein was found to be localized in the nucleus and possessed transcriptional activity. Overexpression of OsRPH1 resulted in a decrease in plant height and length of internode and leaf sheath as well as other abnormal characters in rice. The length of the second leaf sheath of OsRPH1-overexpressing (OE) plants recovered to that of Kitaake (non-transgenic recipient) in response to exogenous gibberellin A3 (GA3) application. The expression of GA biosynthesis genes (OsGA20ox1-OsGA20ox4, OsGA3ox1, and OsGA3ox2) was significantly downregulated, whereas that of GA inactivation genes (OsGA2ox7, OsGA2ox9, and OsGA2ox10) was significantly upregulated in OsRPH1-OE plants. Endogenous bioactive GA contents significantly decreased in OsRPH1-OE plants. OsRPH1 interacted with a blue light receptor, OsCRY1b, in a blue light-dependent manner. Taken together, our results demonstrate that OsRPH...Continue Reading

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Citations

Jul 25, 2021·International Journal of Molecular Sciences·Se Eun JungJu-Kon Kim
Jul 31, 2021·Plant Physiology and Biochemistry : PPB·Shuangzhan HuangXinglin Du
Aug 20, 2021·Frontiers in Plant Science·Hua ZhongYangsheng Li
Aug 28, 2021·International Journal of Molecular Sciences·Shuangzhan HuangXinglin Du

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Methods Mentioned

BETA
PCR
Fluorescence
Two-Hybrid
transgenic

Software Mentioned

DNAMAN
BLASTP
MEGA

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