A novel application of dorsal window chambers: repetitive imaging of tumor-associated lymphatics

Microvascular Research
Jon-Vidar GaustadEinar K Rofstad

Abstract

The purpose of this study was to establish a lymphangiography assay allowing repetitive imaging of tumor-associated lymphatics, and to investigate potential associations between tumor-induced changes in pre-existing lymphatics and tumor angiogenesis. A-07-GFP human melanoma xenografts grown in dorsal window chambers were used as preclinical tumor model. Lymphatics were visualized after multiple intradermal injections of 155 kDa tetramethylrhodamine isothiocyanate dextran outside the window chambers, and tumor vasculature was assessed from high-resolution transillumination images. Lymphangiography was performed thrice in window chambers with or without A-07-GFP tumors, with 3-4 days between repetitions. The lymphangiography assay was highly reproducible. A-07-GFP tumors induced dilation of pre-existing lymphatics after the onset of tumor angiogenesis, and the dilation was correlated with tumor size.

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Citations

Oct 19, 2013·Pharmacology & Therapeutics·Lian NarunskyMichal Neeman
Sep 18, 2012·Microvascular Research·Constantino Carlos Reyes-AldasoroGillian M Tozer
Jul 31, 2013·Current Opinion in Cell Biology·Stephanie AlexanderPeter Friedl
Oct 12, 2017·Journal of Translational Medicine·Ruixia HuangEinar K Rofstad
Feb 20, 2021·European Journal of Nuclear Medicine and Molecular Imaging·Jon-Vidar GaustadEinar K Rofstad

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