A novel approach for identification and measurement of hemoglobin adducts with 1,2,3,4-diepoxybutane by liquid chromatography/electrospray ionisation mass spectrometry and matrix-assisted laser desorption/ionisation tandem mass spectrometry

Rapid Communications in Mass Spectrometry : RCM
A BasileA Malorni

Abstract

The structural characterisation of the adducts formed by in vitro interaction of hemoglobin (Hb) with 1,2,3,4-diepoxybutane (DEB), the most reactive 1,3-butadiene (BD) metabolite, was obtained by liquid chromatography/electrospray ionisation mass spectrometry (LC/ES-MS) analysis of modified tryptic peptides of human hemoglobin chains. The reactive sites of human hemoglobin towards DEB and its hydroxylated derivatives (trihydroxybutyl (THB)-derivatives) were identified through the characterisation of alkylated tryptic peptides by matrix-assisted laser desorption/ionisation tandem mass spectrometry (MALDI-MS/MS). Based on this characterisation, a procedure was set up to measure the Hb-adducts of THB-derivatives by isotope dilution mass spectrometry with the use of a deuterated peptide standard. The results obtained here could permit optimisation of molecular dosimetry of BD-adducts, and extension of the analysis to the biological monitoring of occupational exposure to butadiene.

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Citations

Oct 12, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·M TörnqvistP Rydberg
Nov 25, 2003·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Shufeng Zhou
Dec 12, 2012·Toxicology·Shilpi GoelGunnar Boysen
Nov 18, 2008·Journal of Chromatography. a·Adriana BasileNicola Sannolo
Aug 18, 2005·Rapid Communications in Mass Spectrometry : RCM·Christian H LindhArieh S Cohen
Aug 13, 2003·Rapid Communications in Mass Spectrometry : RCM·Simone CristoniFederico Guidugli
Sep 1, 2004·Journal of Separation Science·Charlotta FredMargareta Törnqvist
Oct 7, 2003·Mass Spectrometry Reviews·Simone Cristoni, Luigi Rossi Bernardi
Apr 1, 2011·Rapid Communications in Mass Spectrometry : RCM·Maria OspinaHubert W Vesper
Nov 7, 2006·Chemico-biological Interactions·Gunnar BoysenJames A Swenberg
Jul 25, 2006·Chemico-biological Interactions·Nadia I GeorgievaJames A Swenberg
Aug 28, 2001·Journal of Mass Spectrometry : JMS
Apr 12, 2002·Rapid Communications in Mass Spectrometry : RCM·Nadia MiragliaNicola Sannolo
Nov 26, 2002·Journal of Mass Spectrometry : JMS·Nadia MiragliaNicola Sannolo
Jun 27, 2019·Rapid Communications in Mass Spectrometry : RCM·Gunnar BoysenArnon Nagler
Jun 23, 2004·Chemical Research in Toxicology·Charlotta FredMargareta Törnqvist
Nov 14, 2021·Archives of Toxicology·Gabriele Sabbioni, Billy W Day

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