A novel approach of virulome based reverse vaccinology for exploring and validating peptide-based vaccine candidates against the most troublesome nosocomial pathogen: Acinetobacter baumannii

Journal of Molecular Graphics & Modelling
Sajjad Ahmad, Syed Sikander Azam

Abstract

Acinetobacter baumannii is one of the major cause of nosocomial infections around the globe. The emergence of hyper-virulent strains of the pathogen greatly narrows down therapeutic options for patients infected with this red alert superbug. Development of a peptide-based vaccine can offers an alternative, attractive, and cost-effective remedy for multidrug-resistant A. baumannii associated complications. Herein, we introduced a novel virulome based Reverse Vaccinology for screening peptide based vaccine candidates against A. baumannii and its validation using a negative control. The pipeline screened "FYLNDQPVS" of polysaccharide export outer membrane protein (EpsA) and "LQNNTRRMK" of chaperone-usher pathway protein B (CsuB) as broad-spectrum peptides for induction of targeted immune responses. The 9-mer epitope of both proteins was rendered virulent, antigenic, non-allergen, and highly conserved among thirty-four completely annotated strains. Interactome examination unravels peptides protein direct and indirect interactions with biological significant pathways, essential for A. baumannii pathogenesis and survival. Protein-peptide docking aids in addition by unveiling deep binding of the epitopes in the active site of the most...Continue Reading

Citations

May 19, 2020·International Journal of Peptide Research and Therapeutics·Iraj RasooliShakiba Darvish Alipour Astaneh
Dec 9, 2020·FEMS Microbiology Reviews·Mireia López-SilesMichael J McConnell
May 4, 2021·Frontiers in Immunology·Michael J McConnell, Antonio J Martín-Galiano
May 7, 2021·FEMS Microbiology Reviews·Mireia López-SilesMichael J McConnell
Jun 10, 2021·Human Vaccines & Immunotherapeutics·NorAziyah Mat RahimSazaly AbuBakar

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