Abstract
Biofilm microorganisms are known to have a much higher tolerance to antimicrobials compared to their planktonic equivalents. Therefore, traditional antimicrobial susceptibility testing may not extrapolate to clinical treatment of infections of biofilm origin, and as a result, there is a need to not only develop antimicrobials with antibiofilm activity, but also suitable in vitro testing methods for their evaluation. In this study, we report on a novel method of antibiofilm testing using a thermo-reversible matrix (poloxamer 407), coupled with live/dead staining of bacteria cultured from the matrix. Pseudomonas aeruginosa (NCIMB 8626) was cultured in medium containing poloxamer 407 at 37°C for 24 hours to generate biofilms. The preparation was cooled to liquefy the poloxamer and allow recovery of the biofilm cells, which were then stained with SYTO9 to determine viability following exposure to four antimicrobials: polyhexanide, octenadine dihydrochloride, povidone-iodine and silver carbonate. Over an 8-minute time period, fluorescence levels were spectrophotometrically measured and compared with bacterial controls, cultured in the absence of poloxamer and without antimicrobial. Untreated cells showed no reduction in viability ov...Continue Reading
References
Oct 20, 1998·American Journal of Surgery·W K StadelmannG R Tobin
Jan 1, 1999·Journal of Applied Microbiology·P GilbertP Wood
May 15, 1999·Journal of Clinical Microbiology·H CeriA Buret
May 21, 1999·Science·J W CostertonE P Greenberg
Jun 27, 2006·Plastic and Reconstructive Surgery·Thomas A MustoeOliver Kloeters
Feb 17, 2007·Annals of Clinical Microbiology and Antimicrobials·Abi L ClutterbuckSteven L Percival
Jul 27, 2007·International Wound Journal·Steven L PercivalJayne Dolman
Dec 19, 2007·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Garth A JamesPhilip S Stewart
Jun 25, 2009·Journal of Applied Microbiology·R M S Thorn, J Greenman
Dec 15, 2010·Critical Care Clinics·Marta UlldemolinsJordi Rello
Sep 19, 2012·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Steven L PercivalJohn W Costerton
Mar 20, 2013·BioMed Research International·Aleksandra TaraszkiewiczJoanna Nakonieczna
May 3, 2013·APMIS. Supplementum·Thomas Bjarnsholt
Oct 23, 2013·International Journal of Molecular Sciences·Qing Wei, Luyan Z Ma
Dec 27, 2013·BioMed Research International·Julia HurlerNataša Škalko-Basnet
Jan 7, 2014·Journal of Microbiological Methods·Hala AlmshawitDanilla Grando
Feb 15, 2014·Advances in Wound Care·Ge ZhaoJohn E Olerud
Apr 29, 2014·The Journal of Pathology·Sashwati RoyChandan K Sen
Jun 1, 2014·Journal of Microbiological Methods·J KuceraP Klein
Oct 1, 2014·Journal of Endodontics·Maria Teresa Arias-MolizCarmen María Ferrer-Luque