A novel approach to chemical microarray using ketone-modified macromolecular scaffolds: application in micro cell-adhesion assay

Molecular Diversity
Qingchai XuKit S. Lam

Abstract

This paper describes a novel strategy for the preparation of chemical microarrays using macro-molecular scaffolds. The macromolecular scaffolds are first functionalized with ketone groups and compounds of interest containing an aminooxy group are conjugated onto the ketone-modified scaffolds through a chemoselective oxime ligation. The conjugate mixtures are then spotted directly onto a plastic or glass surface to form compound microarrays. Because a constant amount of scaffold is used in the presence of excess compound in the ligation reaction, the amount of compound actually immobilized per microarray spot is constant and dependent on the scaffold concentration. Using this approach, 60 different peptides were ligated to human serum albumin or agarose scaffolds, and the peptide conjugates subsequently printed on glass or polystyrene surface to form microarrays. These peptide microarrays were subsequently evaluated and optimized for binding of Jurkat leukemic cancer cells.

Citations

Feb 3, 2005·The Journal of Peptide Research : Official Journal of the American Peptide Society·X WangK S Lam
Apr 23, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Katsushi AmanoM Eric Gershwin
Sep 10, 2005·Expert Review of Molecular Diagnostics·Brigitte Angres
Aug 2, 2006·Molecular BioSystems·Pappanaicken R Kumaresan, Kit S Lam
Mar 31, 2015·Journal of Laboratory Automation·Yung-Shin Sun
Nov 11, 2008·Langmuir : the ACS Journal of Surfaces and Colloids·Y S SunK S Lam
May 23, 2008·Bioconjugate Chemistry·Pappanaicken R KumaresanKit S Lam
Apr 13, 2013·Bioconjugate Chemistry·Ouafâa El-Mahdi, Oleg Melnyk

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