A novel approach to decalcification in histopathology laboratory: An adaptation from the Hammersmith protocol

Indian Journal of Pathology & Microbiology
Prita PradhanArikkotil Jyothish

Abstract

Utility of modified Hammersmith protocol in the deacalcification and/or softening of tissues and samples in a histopathology laboratory were studied. The object of the study was to prepare a novel method for softening/decalcifying tissue for histopathology. All the hard tissues received in the histopathology section were received in 10% neutral buffered formalin and then placed in freshly prepared combination of 10 mL of concentrated formaldehyde and 5 mL of 10% formic acid in 85 mL distilled water was used for decalcification. The tissue was checked for evidence of adequate decalcification/softening every 6 hours. Those which were decalcified/softened were sent for routine tissue processing and staining, while those which were not, were again placed in formalin. The process was repeated until the tissue was ready for further processing. The routine sections of these slides were reviewed for morphology and stain quality along with special stains and immunohistochemistry performed. The time taken for decalcification, the variables most likely to affect decalcification, the morphology and staining characteristics were documented. Statistical analysis was done to determine the effect of softening/decalcification process on each va...Continue Reading

References

Oct 3, 2002·American Journal of Respiratory and Critical Care Medicine·Hajime FukunagaTokuhiro Ishihara
Aug 27, 2016·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Willemijne A M E SchrijverPaul J van Diest

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