A novel approach to in situ characterization of pancreatic beta-cells

Pflügers Archiv : European journal of physiology
S Speier, Marjan Rupnik

Abstract

The tissue-slice technique has enabled major insights into neural and neuroendocrine physiology. Our aim was to adapt this technique to study the function of the endocrine pancreas. The preparation combines an in situ approach, as in gland perfusion, with a resolution characteristic of electrophysiological studies on single cells. The membrane potential in beta-cells in the slices recorded using the whole-cell patch-clamp was close to the calculated reversal potential for K+. With sufficient ATP in the recording pipette the beta-cells depolarized rapidly on exposure to an increased glucose concentration or stimulation with tolbutamide. The cells preserved bursting and spiking capacity for tens of minutes despite the whole-cell dialysis. In addition, the voltage clamp was used to monitor the changes in the membrane capacitance and to allow correlation of the electrical activity and the cytosolic calcium changes. The pancreatic tissue slice preparation is a novel method for studying the function of the beta- and other pancreatic endocrine and exocrine cells under near-physiological conditions.

References

Jan 1, 1992·Pflügers Archiv : European journal of physiology·K D Gillis, S Misler
Mar 1, 1992·Pflügers Archiv : European journal of physiology·R Schneggenburger, J López-Barneo
Jan 1, 1986·Advances in Experimental Medicine and Biology·G Trube, P Rorsman
Feb 1, 1988·Pflügers Archiv : European journal of physiology·M Pusch, E Neher
Jan 1, 1989·Progress in Biophysics and Molecular Biology·F M Ashcroft, P Rorsman
Feb 1, 1973·The Biochemical Journal·S J AshcroftP J Randle
Sep 1, 1970·The Journal of Physiology·P M Dean, E K Matthews
Nov 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·E Neher, A Marty
Dec 7, 2002·Diabetes/metabolism Research and Reviews·Susanne G Straub, Geoffrey W G Sharp

❮ Previous
Next ❯

Citations

Nov 23, 2011·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Shi-Bing YangLily Yeh Jan
Jan 9, 2013·The Journal of Membrane Biology·Na ZouYing Shen
Apr 3, 2013·Purinergic Signalling·Geoffrey Burnstock, Ivana Novak
Mar 11, 2008·Nature Medicine·Stephan SpeierPer-Olof Berggren
Dec 14, 2012·Proceedings of the National Academy of Sciences of the United States of America·Rayner Rodriguez-DiazAlejandro Caicedo
May 31, 2007·The Journal of General Physiology·Tobias RoseMarjan Rupnik
Nov 6, 2008·Acta Physiologica·M Rupnik
Mar 8, 2013·PLoS Computational Biology·Andraž StožerDean Korošak
Aug 21, 2014·Chronobiology International·Mareike Hildegard MüllerHorst-Werner Korf
Aug 10, 2013·Diabetes·Anna IvanovaMichele Solimena
Jan 10, 2018·Frontiers in Physiology·Marko GosakMarko Marhl
Dec 20, 2018·Journal of Cellular Physiology·Mohammad Javad Saeedi BorujeniAmid Yazdani
May 31, 2008·The Journal of Physiology·Lena EliassonPatrik Rorsman
Sep 18, 2013·Diabetes, Obesity & Metabolism·M H Abdulreda, P-O Berggren
Dec 8, 2017·Physiological Reviews·Patrik Rorsman, Frances M Ashcroft
Nov 17, 2010·The Journal of Physiology·Ya-Chi HuangHerbert Y Gaisano
Feb 9, 2018·Frontiers in Physiology·Dean Korošak, Marjan Slak Rupnik
Oct 18, 2019·Frontiers in Physiology·Dean Korošak, Marjan Slak Rupnik
Aug 10, 2019·Frontiers in Physiology·Eleonóra GálViktória Venglovecz
Jul 1, 2020·Nature Communications·Mirza Muhammad Fahd QadirJuan Domínguez-Bendala
Jun 22, 2011·The Journal of Biological Chemistry·Slavena A MandicChristina Bark
Feb 2, 2021·Frontiers in Endocrinology·Luciana Mateus Gonçalves, Joana Almaça
May 1, 2020·Biophysical Journal·Boris PodobnikMarko Jusup
Jul 23, 2011·Physiological Research·J DolenšekM S Rupnik
Jul 19, 2011·Progress in Biophysics and Molecular Biology·Patrik RorsmanSven Gopel
May 20, 2021·Trends in Endocrinology and Metabolism : TEM·Juan Domínguez-BendalaRicardo Luis Pastori

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.