A novel approach to monitor stress-induced physiological responses in immobilized microorganisms

Applied Microbiology and Biotechnology
Saúl AlonsoMario Díaz

Abstract

Microbial cell immobilization has long been considered as a potential bioprocessing strategy to increase both microorganisms' tolerance and fitness in fermentation systems. To date, little emphasis has been put on how the entrapped cells respond to the bioprocessing stresses encountered during the cultivation. The present work presents for the first time a methodology to decipher the real health status of the entrapped microorganisms by combining multiparameter flow cytometry with confocal fluorescence microscopy as monitoring tools. Comparison between resting free and immobilized cell-based systems enabled to characterize the spatial-temporal physiological response of entrapped Pseudomonas taetrolens cells during lactobionic acid production in submerged cultivation. Whereas cellular leakage from beads led to planktonic cells that faced a progressive loss of membrane integrity, immobilized cells underwent a prompt stress-induced physiological response featured by the predominance of cellular damaging. Moreover, visualization without matrix de-entrapment through confocal fluorescence microscopy revealed the overtime formation of cellular micro-colonies inside the beads. These micro-colonies comprised a shell made of dead cells, ...Continue Reading

References

Feb 8, 2008·Applied Microbiology and Biotechnology·Mei ChiaWon Jae Choi
Mar 7, 2008·Langmuir : the ACS Journal of Surfaces and Colloids·Zhiyong SuoDavid W Pascual
Jul 20, 1996·Biotechnology and Bioengineering·M DíazL A García
Aug 21, 2008·Journal of Industrial Microbiology & Biotechnology·Mahmoud AbouseoudRachida Maachi
Jul 11, 2012·Applied Microbiology and Biotechnology·Saúl AlonsoMario Díaz
Oct 30, 2012·Applied Microbiology and Biotechnology·Johan O WestmanMohammad J Taherzadeh
Oct 31, 2012·International Journal of Molecular Sciences·Johan O WestmanMohammad J Taherzadeh
Mar 6, 2013·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·F Martin-DejardinO Chambin
May 9, 2013·Biotechnology Advances·Saúl AlonsoMario Díaz

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