A novel approach to non-biased systematic random sampling: a stereologic estimate of Purkinje cells in the human cerebellum.

Brain Research
Rajiv M AgashiwalaDaniel P Perl

Abstract

Non-biased systematic sampling using the principles of stereology provides accurate quantitative estimates of objects within neuroanatomic structures. However, the basic principles of stereology are not optimally suited for counting objects that selectively exist within a limited but complex and convoluted portion of the sample, such as occurs when counting cerebellar Purkinje cells. In an effort to quantify Purkinje cells in association with certain neurodegenerative disorders, we developed a new method for stereologic sampling of the cerebellar cortex, involving calculating the volume of the cerebellar tissues, identifying and isolating the Purkinje cell layer and using this information to extrapolate non-biased systematic sampling data to estimate the total number of Purkinje cells in the tissues. Using this approach, we counted Purkinje cells in the right cerebella of four human male control specimens, aged 41, 67, 70 and 84 years, and estimated the total Purkinje cell number for the four entire cerebella to be 27.03, 19.74, 20.44 and 22.03 million cells, respectively. The precision of the method is seen when comparing the density of the cells within the tissue: 266,274, 173,166, 167,603 and 183,575 cells/cm3, respectively....Continue Reading

References

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Citations

Mar 4, 2014·PloS One·Jerry SkefosMargaret Bauman
May 30, 2013·Brain Structure & Function·Maren C KiesslingChristoph Schmitz
Dec 5, 2012·International Journal of Radiation Biology·Ayşegül AkarGastone Castellani
Aug 24, 2010·Neurobiology of Aging·Kjeld AndersenBente Pakkenberg
May 18, 2016·The Journal of Comparative Neurology·Christopher S von BartheldSuzana Herculano-Houzel
Jan 24, 2018·Frontiers in Neuroanatomy·Anna FichtlMaren C Kiessling
Dec 2, 2020·Movement Disorders : Official Journal of the Movement Disorder Society·Whitney G HartstonePhyllis L Faust

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