PMID: 9551087Apr 29, 1998Paper

A novel Arabidopsis thaliana protein protects tumor cells from tumor necrosis factor-induced apoptosis

Biochimica Et Biophysica Acta
R U JänickeA Kush

Abstract

Recently we have cloned and characterized a novel, oxidative stress-induced Arabidopsis thaliana gene (oxy5), and showed that expression of oxy5 protects bacterial cells from death caused by oxidative stress. As oxidative stress is one pathway of TNF cytotoxicity, we investigated whether the encoded protein could also protect human tumor cells from TNF killing. We stably transfected the oxy5 gene into TNF-sensitive HeLa D98 cells (D98/O.5), and found that all examined transfectants were highly TNF-resistant in the absence of the protein synthesis inhibitor cycloheximide. The acquired TNF resistance of these clones was accompanied by a sharp decrease in the intracellular formation of reactive oxygen species, suggesting the activation of antioxidant enzymes like superoxide dismutases (SODs). Indeed, D98/O.5 clones showed an increased manganous superoxide dismutase (MnSOD) mRNA and protein expression in the absence or presence of TNF stimulation, whereas the expression of the Cu/ZnSOD was not affected. Furthermore, the elevated MnSOD expression in the D98/O.5 clones correlated well with an increased antioxidative activity, which was specifically due to MnSOD as measured by the suppression of xanthine oxidase. Our results demonstra...Continue Reading

References

Nov 6, 1992·Science·C E Creutz
Apr 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·J D Croxtall, R J Flower
Jan 1, 1988·Free Radical Biology & Medicine·H M Hassan
May 1, 1986·Nature·R B Pepinsky, L K Sinclair
Oct 22, 1993·Cell·D M HockenberyS J Korsmeyer
Apr 27, 1995·Nature·M D Jacobson, M C Raff
Aug 29, 1995·Proceedings of the National Academy of Sciences of the United States of America·V GoossensW Fiers
Jan 1, 1994·Immunology Today·T M Buttke, P A Sandstrom
Feb 1, 1993·Current Opinion in Immunology·N J Goulding, P M Guyre
May 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·W G HoeckR A Heller
Oct 1, 1996·Molecular and Cellular Biology·R U JänickeA G Porter
Oct 1, 1996·Proceedings of the National Academy of Sciences of the United States of America·X GidrolA K Kush
Nov 1, 1996·Science·M Barinaga
Jul 1, 1997·Genes & Development·Y YangD F Klessig

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Citations

Jun 2, 2009·Plant Physiology·Dorota Konopka-PostupolskaJacek Hennig
Sep 13, 2005·Biochemical and Biophysical Research Communications·Karolina M GoreckaSlawomir Pikula
Mar 11, 2010·Plant Signaling & Behavior·Greg ClarkStanley Roux
Mar 26, 2013·Journal of Genetics and Genomics = Yi Chuan Xue Bao·Chuan-Ming XieYi Sun
Jul 31, 2012·Gastroenterology·Etienne PatinUNKNOWN French ANRS HC EP 26 Genoscan Study Group
Apr 20, 2017·International Journal of Molecular Sciences·Dorota Konopka-Postupolska, Greg Clark
Jan 5, 2001·Journal of Experimental Botany·C I Ullrich, R Aloni

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