A novel arsenic methyltransferase gene of Westerdykella aurantiaca isolated from arsenic contaminated soil: phylogenetic, physiological, and biochemical studies and its role in arsenic bioremediation

Metallomics : Integrated Biometal Science
Shikha VermaDebasis Chakrabarty

Abstract

Elevated arsenic concentration in the environment and agricultural soil is a serious concern to crop production and human health. Among different detoxification mechanisms, the methylation of arsenic is a widespread phenomenon in nature. A number of microorganisms are able to methylate arsenic, but less is known about the arsenic metabolism in fungi. We identified a novel arsenic methyltransferase (WaarsM) gene from a soil fungus, Westerdykella aurantiaca. WaarsM showed sequence homology with all known arsenic methyltransferases having three conserved SAM binding motifs. The expression of WaarsM enhanced arsenic resistance in E. coli (Δars) and S. cerevisiae (Δacr2) strains by biomethylation and required endogenous reductants, preferably GSH, for methyltransferase activity. The purified WaarsM catalyzes the production of methylated arsenicals from both AsIII and AsV, and also displays AsV reductase activity. It displayed higher methyltransferase activity and lower KM 0.1945 ± 0.021 mM and KM 0.4034 ± 0.078 mM for AsIII and AsV, respectively. S. cerevisiae (Δacr2) cells expressing WaarsM produced 2.2 ppm volatile arsenic and 0.64 ppm DMA(v) with 0.58 ppm volatile arsenicals when exposed to 20 ppm AsV and 2 ppm AsIII, respectivel...Continue Reading

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Citations

Feb 6, 2017·Environmental Pollution·Pratibha TripathiChandra S Nautiyal
Apr 24, 2018·Frontiers in Plant Science·Shalini Tiwari, Charu Lata
Jan 23, 2019·Applied Microbiology and Biotechnology·Changdong KeSuping Yang
Mar 24, 2020·Ecotoxicology and Environmental Safety·Jasvinder KaurSuchi Srivastava
Mar 24, 2020·Ecotoxicology and Environmental Safety·Pankaj Kumar VermaDebasis Chakrabarty

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