A Novel Arthropod Host of Brucellosis in the Arid Steppe Ecosystem

Frontiers in Veterinary Science
Tianpeng Huang Geriletu

Abstract

Brucellosis is a severe public health problem in the Inner Mongolia regions of China. The recent prevalence of brucellosis outbreaks may be attributed to an increase in the activity of ticks and other air-borne vectors. Dermacentor nuttalli (D. nuttalli) is a native tick species of Inner Mongolia; similar to other tick species, D. nuttalli carries a variety of pathogens that can be transmitted to a wide range of animals. In this study, we have investigated the potential of D. nuttalli in transmitting brucellosis. From 2015 to 2019, 2,256 ticks were collected from 23 different pastoral areas of Hulun Buir. Brucellosis pathogen was detected using DNA extracted from different developmental stages of ticks. Salivary gland and midgut tissue samples were used as templates to amplify Brucella Bscp31 gene (Brucella genus-specific gene) by using TaqMan Real-Time polymerase chain reaction (PCR). To detect the presence of Bscp31 protein, which is specific to Brucella spp., in the midgut and salivary gland tissues of D. nuttalli, Western blotting and immunofluorescence were performed. Additionally, Brucella spp. were isolated using a culture medium. Tick samples were identified as D. nuttalli. Different percentages of Brucella genus-specif...Continue Reading

References

Nov 5, 2002·Veterinary Microbiology·Shang DeqiuYin Jiming
Aug 18, 2005·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·M I Queipo-OrtuñoP Morata
Jan 27, 2010·Experimental & Applied Acarology·Ze ChenJingze Liu
Dec 3, 2010·Emerging Infectious Diseases·Wen-Yi ZhangWu-Chun Cao
Sep 20, 2011·Vector Borne and Zoonotic Diseases·Zou YaxueChen Lifeng
Sep 24, 2011·Annual Review of Microbiology·Vidya L AtluriRenée M Tsolis
Aug 10, 2012·Recent Patents on Anti-infective Drug Discovery·Alfonso J Rodríguez-Morales
Oct 18, 2013·Molecular Biology and Evolution·Koichiro TamuraSudhir Kumar
Mar 8, 2014·Parasitology Research·Nina V KulakovaSergei I Belikov
Apr 22, 2015·The American Journal of Pathology·Paul de FigueiredoL Garry Adams
Aug 1, 2015·Parasitology International·Bumduuren TuvshintulgaNaoaki Yokoyama
Sep 1, 2016·Revista brasileira de parasitologia veterinária = Brazilian journal of veterinary parasitology : Órgão Oficial do Colégio Brasileiro de Parasitologia Veterinária·Rodrigo Leite SoaresCarlos Alberto do Nascimento Ramos
Jan 19, 2017·Emerging Infectious Diseases·Shengjie LaiHongjie Yu
Feb 6, 2017·Parasites & Vectors·Bazartseren BoldbaatarGregory C Gray
Apr 9, 2018·Ticks and Tick-borne Diseases·Qian WangYuanzhi Wang
Apr 4, 2019·Parasites & Vectors·Emilie LejalThomas Pollet

❮ Previous
Next ❯

Methods Mentioned

BETA
Polymerase Chain
PCR
electrophoresis
biotyping

Software Mentioned

Clustal
MEGA
GraphPad Prism

Related Concepts

Related Feeds

Brucellosis (ASM)

Brucellosis is a bacterial infection caused by members of the genus brucella and remains one of the world's major zoonotic diseases. Discover the latest research on Brucellosis here.

Brucellosis

Brucellosis is a bacterial infection caused by members of the genus brucella and remains one of the world's major zoonotic diseases. Discover the latest research on Brucellosis here.