A novel assay reveals hygrotactic behavior in Drosophila

PloS One
Feiteng Ji, Yan Zhu

Abstract

Humidity is one of the most important factors that determines the geographical distribution and survival of terrestrial animals. The ability to detect variation in humidity is conserved across many species. Here, we established a novel behavioral assay that revealed the thirsty Drosophila exhibits strong hygrotactic behavior, and it can locate water by detecting humidity gradient. In addition, exposure to high levels of moisture was sufficient to elicit proboscis extension reflex behavior in thirsty flies. Furthermore, we found that the third antennal segment was necessary for hygrotactic behavior in thirsty flies, while arista was required for the avoidance of moist air in hydrated flies. These results indicated that two types of hygroreceptor cells exist in Drosophila: one located in the third antennal segment that mediates hygrotactic behavior in thirst status, and the other located in arista which is responsible for the aversive behavior toward moist air in hydration status. Using a neural silencing screen, we demonstrated that synaptic output from the mushroom body α/β surface and posterior neurons was required for both hygrotactic behavior and moisture-aversive behavior.

References

Apr 28, 1988·Nature·J A FischerM Ptashne
Jul 1, 1993·Neuron·R L Davis
Jun 11, 1996·Proceedings of the National Academy of Sciences of the United States of America·O Sayeed, S Benzer
Feb 1, 1996·Current Biology : CB·R L Davis, K A Han
May 1, 1997·Journal of Neurobiology·R F StockerJ S de Belle
Jan 12, 1952·Nature·V PERTTUNEN, H ERKKILA
Jan 1, 1997·Annual Review of Entomology·M F Land
Jun 24, 2004·Cell·Zuoren WangKristin Scott
Sep 16, 2005·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·C Andrea YaoJohn R Carlson
Jan 18, 2006·Proceedings of the National Academy of Sciences of the United States of America·Tsuyoshi Inoshita, Teiichi Tanimura
Mar 27, 2007·Cell·Hui-Hao LinAnn-Shyn Chiang
May 18, 2007·Annual Review of Neuroscience·Leslie B Vosshall, Reinhard F Stocker
Jan 18, 2008·Kidney International·R Sharif-NaeiniC W Bourque
Apr 9, 2008·The Journal of Comparative Neurology·Nobuaki K TanakaKei Ito
May 30, 2008·Nature Reviews. Neuroscience·Charles W Bourque
Jan 14, 2009·Journal of Neurogenetics·Yoshinori AsoHiromu Tanimoto
Dec 8, 2009·Trends in Neurosciences·Masha Prager-Khoutorsky, Charles W Bourque
Apr 7, 2010·Nature·Peter CameronKristin Scott
Apr 9, 2010·Journal of Neurophysiology·H Tichy, W Kallina
May 7, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Zijing ChenZuoren Wang
Aug 20, 2011·Journal of the American Society of Nephrology : JASN·Stine Falsig PedersenElse K Hoffmann
Jun 15, 2013·Science·Yali V ZhangCraig Montell
Jul 31, 2013·Proceedings of the National Academy of Sciences of the United States of America·Wei ZhangYuh Nung Jan
May 21, 2014·Proceedings of the National Academy of Sciences of the United States of America·Joshua RussellJonathan T Pierce-Shimomura

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Citations

May 21, 2016·Frontiers in Physiology·Makoto MizunamiFumio Yokohari
May 11, 2016·Current Biology : CB·Anders EnjinMarcus C Stensmyr
Sep 1, 2018·Nature Communications·J Felipe Méndez-ValderramaT A Arias
Jan 31, 2021·Cell and Tissue Research·K P SijuIlona C Grunwald Kadow

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Methods Mentioned

BETA
transgenic

Software Mentioned

MATLAB

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