A Novel Autoantibody against Plexin D1 in Patients with Neuropathic Pain

Annals of Neurology
Takayuki FujiiJun-Ichi Kira

Abstract

To identify novel autoantibodies for neuropathic pain (NeP). We screened autoantibodies that selectively bind to mouse unmyelinated C-fiber type dorsal root ganglion (DRG) neurons using tissue-based indirect immunofluorescence assays (IFA) with sera from 110 NeP patients with various inflammatory and allergic neurologic diseases or other neuropathies, and 50 controls without NeP including 20 healthy subjects and 30 patients with neurodegenerative diseases or systemic inflammatory diseases. IgG purified from IFA-positive patients' sera was subjected to Western blotting (WB) and immunoprecipitation (IP) using mouse DRG lysates. Immunoprecipitates were analyzed by liquid chromatography tandem mass spectrometry (LC-MS/MS) to identify target autoantigens. Antiunmyelinated C-fiber type DRG neuron antibodies were more frequent in patients with NeP than non-NeP subjects (10% vs 0%; p < 0.05). These autoantibodies were all from the IgG2 subclass and colocalized mostly with isolectin B4- and P2X3-positive pain-conducting small neurons but not with S100β-positive myelinated neurons. WB revealed a common immunoreactive band (approximately 220kDa). IP and LC-MS/MS studies identified plexin D1 as a target autoantigen. Immunoadsorption tests ...Continue Reading

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Citations

Jan 11, 2020·Frontiers in Neurology·Takayuki FujiiJun-Ichi Kira
Aug 21, 2020·Neurology. Neuroimmunology and Neuroinflammation·Josep Dalmau
Jun 27, 2020·Neurology. Neuroimmunology and Neuroinflammation·Takayuki FujiiJun-Ichi Kira
Nov 24, 2020·Neurologic Clinics·Lawrence A Zeidman
Jun 9, 2021·Neurology. Neuroimmunology and Neuroinflammation·Takayuki FujiiJun-Ichi Kira
Nov 12, 2021·Annals of Neurology·Amanda C Y ChanAnselm Mak

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