A novel automated device for rapid nucleic acid extraction utilizing a zigzag motion of magnetic silica beads

Analytica Chimica Acta
Akemi YamaguchiYasunori Saito

Abstract

We report a novel automated device for nucleic acid extraction, which consists of a mechanical control system and a disposable cassette. The cassette is composed of a bottle, a capillary tube, and a chamber. After sample injection in the bottle, the sample is lysed, and nucleic acids are adsorbed on the surface of magnetic silica beads. These magnetic beads are transported and are vibrated through the washing reagents in the capillary tube under the control of the mechanical control system, and thus, the nucleic acid is purified without centrifugation. The purified nucleic acid is automatically extracted in 3 min for the polymerase chain reaction (PCR). The nucleic acid extraction is dependent on the transport speed and the vibration frequency of the magnetic beads, and optimizing these two parameters provided better PCR efficiency than the conventional manual procedure. There was no difference between the detection limits of our novel device and that of the conventional manual procedure. We have already developed the droplet-PCR machine, which can amplify and detect specific nucleic acids rapidly and automatically. Connecting the droplet-PCR machine to our novel automated extraction device enables PCR analysis within 15 min, a...Continue Reading

References

Feb 14, 1992·Analytical Biochemistry·R P AldertonS Beck
Feb 7, 2001·Journal of Clinical Microbiology·W A Al-Soud, P Rådström
Apr 12, 2003·Biotechnology and Applied Biochemistry·Heather N EndresMark R Etzel
Apr 26, 2003·Analytical Chemistry·Michael C BreadmoreJames P Landers
Dec 13, 2006·Proceedings of the National Academy of Sciences of the United States of America·Christopher J EasleyJames P Landers
Jul 16, 2010·Biomedical Microdevices·Yi ZhangTza-Huei Wang
Oct 13, 2010·Lab on a Chip·Marc KarleFelix von Stetten
May 12, 2011·Analytical Chemistry·Gabriela R M DuarteJames P Landers
Nov 15, 2011·Analytical Chemistry·Lewis A MarshallJuan G Santiago
Jul 4, 2012·Journal of Applied Microbiology·C SchraderR Johne
Jul 4, 2012·Clinica Chimica Acta; International Journal of Clinical Chemistry·Kazuyuki MatsudaTakayuki Honda
Sep 29, 2012·Lab on a Chip·Jaime J BenítezHarold G Craighead
May 21, 2013·Clinica Chimica Acta; International Journal of Clinical Chemistry·Chiaki TairaTakayuki Honda
Aug 15, 2013·Analytical Chemistry·Peter C ThomasDavid J Beebe

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.