A novel Bacillus thuringiensis (PS149B1) containing a Cry34Ab1/Cry35Ab1 binary toxin specific for the western corn rootworm Diabrotica virgifera virgifera LeConte forms ion channels in lipid membranes

Biochemistry
L MassonJean-Louis Schwartz

Abstract

The binary Bacillus thuringiensis PS149B1 insecticidal crystal (Cry) protein is comprised of two components, Cry34Ab1, a 14-kDa protein, and Cry35Ab1, a 44-kDa protein, the combination of which forms a novel binary toxin active on western corn rootworm larvae. The permeabilizing behavior of the native binary toxin and its two individual components expressed as recombinant proteins was studied using calcein efflux determination in liposomes and by ion channel activity measurements in planar lipid bilayers (PLBs). Data obtained with solubilized native PS149B1 binary protein revealed it to be a pore-forming toxin that can permeabilize liposomes and form ion channels ( approximately 300-900 pS) in PLBs at pH 5.5 but not pH 9.0. The 14-kDa component of the toxin also formed ion channels ( approximately 15-300 pS) at pH 5.5 but did not insert easily in PLBs. While the 44-kDa moiety did seldomly form resolvable ion channels ( approximately 15-750 pS) in PLBs, it did destabilize the membranes. It showed pH-dependent truncation to a stable 40-kDa protein. The purified 40-kDa truncated product formed channels ( approximately 10-450 pS) in PLBs at pH 5.5. At that same pH, while a 3:1 molar mixture (14:44 kDa) of the individual components ...Continue Reading

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Citations

May 24, 2012·Journal of Invertebrate Pathology·Vincent VachonJean-Louis Schwartz
Oct 23, 2012·Bioscience Reports·Victor V Lemeshko, Sergio Orduz
Aug 3, 2016·Journal of Invertebrate Pathology·Colin Berry, Neil Crickmore
Aug 3, 2016·Journal of Invertebrate Pathology·Kenneth E NarvaRod Herman
May 24, 2013·Microbiology and Molecular Biology Reviews : MMBR·Ferdinand C O LosAdam J Ratner
Jul 25, 2007·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Gareth W JonesColin Berry
Apr 11, 2019·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Yutao Xiao, Kongming Wu
Sep 25, 2020·Journal of Invertebrate Pathology·Anastasija PanevskaKristina Sepčić

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